Determination of antioxidant activity using oxidative damage to plasmid DNA: pursuit of solvent optimization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F13%3A43871809" target="_blank" >RIV/62157124:16370/13:43871809 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.2478/s11696-013-0334-8" target="_blank" >http://dx.doi.org/10.2478/s11696-013-0334-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/s11696-013-0334-8" target="_blank" >10.2478/s11696-013-0334-8</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of antioxidant activity using oxidative damage to plasmid DNA: pursuit of solvent optimization

Popis výsledku v původním jazyce

Oxidative stress plays a key role in the pathophysiology of many diseases. Hydroxyl radical is the oxidative species most commonly causing damage to cells. The aim of this work was to optimize the method for antioxidant activity determination on a modellipophilic geranylated flavanone, diplacone. This method uses protection of plasmid DNA from oxidation by a hydroxyl radical generated by the Fenton reaction involving oxidation of metal ions using H2O2 and ascorbate. The method was optimized for lipophilic compounds using several solvents and co-solvents. It was found that (2-hydroxypropyl)-beta-cyclodextrin (0.1 mass % aq. sol.) is the best co-solvent for our model lipophilic compound to measure the antioxidant activity by the method presented. Othersolvents, namely dimethyl sulfoxide, Cremophor ELA (R) (0.1 mass % aq. sol.), ethanol, and methanol, were not suitable for the determination of the antioxidant activity by the method described. Tween 80 (0.1 mass % aq. sol.) and a mixture

Název v anglickém jazyce

Determination of antioxidant activity using oxidative damage to plasmid DNA: pursuit of solvent optimization

Popis výsledku anglicky

Oxidative stress plays a key role in the pathophysiology of many diseases. Hydroxyl radical is the oxidative species most commonly causing damage to cells. The aim of this work was to optimize the method for antioxidant activity determination on a modellipophilic geranylated flavanone, diplacone. This method uses protection of plasmid DNA from oxidation by a hydroxyl radical generated by the Fenton reaction involving oxidation of metal ions using H2O2 and ascorbate. The method was optimized for lipophilic compounds using several solvents and co-solvents. It was found that (2-hydroxypropyl)-beta-cyclodextrin (0.1 mass % aq. sol.) is the best co-solvent for our model lipophilic compound to measure the antioxidant activity by the method presented. Othersolvents, namely dimethyl sulfoxide, Cremophor ELA (R) (0.1 mass % aq. sol.), ethanol, and methanol, were not suitable for the determination of the antioxidant activity by the method described. Tween 80 (0.1 mass % aq. sol.) and a mixture

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemical Papers - Chemické Zvesti

ISSN

0366-6352

e-ISSN

—

Svazek periodika

67

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

6

Strana od-do

484-489

Kód UT WoS článku

000314882900002

EID výsledku v databázi Scopus

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