Cucurbitacin B interacts synergistically with antibiotics against Staphylococcus aureus clinical isolates and exhibits antiviral activity against HSV-1.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F17%3A43875735" target="_blank" >RIV/62157124:16370/17:43875735 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41330/17:71041

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.sajb.2016.10.001" target="_blank" >http://dx.doi.org/10.1016/j.sajb.2016.10.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.sajb.2016.10.001" target="_blank" >10.1016/j.sajb.2016.10.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cucurbitacin B interacts synergistically with antibiotics against Staphylococcus aureus clinical isolates and exhibits antiviral activity against HSV-1.

Popis výsledku v původním jazyce

The search for biologically promising compounds from natural sources against microbial diseases remains an important theme in drug discovery to overcome problems with drug-resistant strains. In this study, Cucurbitacin B (Cuc B), an active constituent of Ecballium elaterium L., has been investigated in vitro for its synergy effect with antibiotics against clinical isolates of Staphylococcus aureus (S. aureus) and anti-HSV-1 activity. Broth microdilution method was used to determine the antibacterial activity, while checkerboard assay was used to evaluate the synergy effect according to ?FIC indices. The anti-HSV-1 activity was determined by the plaque number reduction assay, while cytotoxicity was evaluated by MTT assay. In this study, Cuc B exerted direct growth-inhibitory activity against all S. aureus strains tested with MICs values ranging from 0.12 to 0.44 ?g/mL, as well as synergy effect with tetracycline or oxacillin against four of six S. aureus strains tested (?FIC ranging from 0.29 to 0.43). Cuc B showed remarkable anti-HSV-1 activity compared with that of acyclovir with IC50 values of 0.94 and 1.74 ?M, respectively and selectivity indices SI = 127.7 and SI N 132.2, respectively. This study presents Cuc B as a promising therapeutic agent in the development of anti-staphylococcal and anti-HSV-1 drugs.

Název v anglickém jazyce

Cucurbitacin B interacts synergistically with antibiotics against Staphylococcus aureus clinical isolates and exhibits antiviral activity against HSV-1.

Popis výsledku anglicky

The search for biologically promising compounds from natural sources against microbial diseases remains an important theme in drug discovery to overcome problems with drug-resistant strains. In this study, Cucurbitacin B (Cuc B), an active constituent of Ecballium elaterium L., has been investigated in vitro for its synergy effect with antibiotics against clinical isolates of Staphylococcus aureus (S. aureus) and anti-HSV-1 activity. Broth microdilution method was used to determine the antibacterial activity, while checkerboard assay was used to evaluate the synergy effect according to ?FIC indices. The anti-HSV-1 activity was determined by the plaque number reduction assay, while cytotoxicity was evaluated by MTT assay. In this study, Cuc B exerted direct growth-inhibitory activity against all S. aureus strains tested with MICs values ranging from 0.12 to 0.44 ?g/mL, as well as synergy effect with tetracycline or oxacillin against four of six S. aureus strains tested (?FIC ranging from 0.29 to 0.43). Cuc B showed remarkable anti-HSV-1 activity compared with that of acyclovir with IC50 values of 0.94 and 1.74 ?M, respectively and selectivity indices SI = 127.7 and SI N 132.2, respectively. This study presents Cuc B as a promising therapeutic agent in the development of anti-staphylococcal and anti-HSV-1 drugs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

South African Journal of Botany

ISSN

0254-6299

e-ISSN

—

Svazek periodika

108

Číslo periodika v rámci svazku

JAN 2017

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

90-94

Kód UT WoS článku

000390671400009

EID výsledku v databázi Scopus

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