Cytotoxic Activities of Prenylated Phenolic Substances

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cytotoxic Activities of Prenylated Phenolic Substances

Popis výsledku v původním jazyce

Natural prenylated phenolics combine a phenolic skeleton with a lipophilic prenyl side-chain. The-side chain may be modified, mainly by oxidation, and this gives enormous degree of structural variability. Combination of the flavonoid skeleton with a lipophilicity of the prenyl side-chain gives the resultant great potential for bio-activity. Prenylated polyphenolics therefore show wide spectrum of biological effects, including antioxidative, antiphlogistic, antimicrobial, anticancerogenic and estrogenic. Our research is focused mainly on prenylated flavonoids and benzofurans isolated from Paulownia tomentosa and Morus alba, respectively. These compounds showed antibacterial, cytotoxic, and anti-inflammatory activity. The cytotoxic potential of prenylated flavonoids was analysed using a number of cancer cell lines and compared with standards. We tried to evaluate the mechanism of the toxic action using analysis of cell cycle and expression of molecules important for cell cycle control. Furthermore, we tried to perform a screening of potential of prenylated flavonoids affect to alter intercellular communication. Twenty flavonoids isolated from P. tomentosa were investigated to elucidate their ability to revert gap junctional intercellular communication (GJIC) using in vitro mammalian tumorigenic cell line WB-Ha-ras. WB-Ha-ras cells are Ha ras oncogene-transformed rat liver epithelial cells, naturally exerting low level of GJIC. Several investigated flavonoids were found to significantly increase GJIC and restore the crucial control homeostatic mechanism in in vitro WB-Ha-ras cells line, indicating potential chemopreventive and anticancer effects.

Název v anglickém jazyce

Cytotoxic Activities of Prenylated Phenolic Substances

Popis výsledku anglicky

Natural prenylated phenolics combine a phenolic skeleton with a lipophilic prenyl side-chain. The-side chain may be modified, mainly by oxidation, and this gives enormous degree of structural variability. Combination of the flavonoid skeleton with a lipophilicity of the prenyl side-chain gives the resultant great potential for bio-activity. Prenylated polyphenolics therefore show wide spectrum of biological effects, including antioxidative, antiphlogistic, antimicrobial, anticancerogenic and estrogenic. Our research is focused mainly on prenylated flavonoids and benzofurans isolated from Paulownia tomentosa and Morus alba, respectively. These compounds showed antibacterial, cytotoxic, and anti-inflammatory activity. The cytotoxic potential of prenylated flavonoids was analysed using a number of cancer cell lines and compared with standards. We tried to evaluate the mechanism of the toxic action using analysis of cell cycle and expression of molecules important for cell cycle control. Furthermore, we tried to perform a screening of potential of prenylated flavonoids affect to alter intercellular communication. Twenty flavonoids isolated from P. tomentosa were investigated to elucidate their ability to revert gap junctional intercellular communication (GJIC) using in vitro mammalian tumorigenic cell line WB-Ha-ras. WB-Ha-ras cells are Ha ras oncogene-transformed rat liver epithelial cells, naturally exerting low level of GJIC. Several investigated flavonoids were found to significantly increase GJIC and restore the crucial control homeostatic mechanism in in vitro WB-Ha-ras cells line, indicating potential chemopreventive and anticancer effects.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

International symposium on natural products in cancer prevention and therapy: Trends in Methods and Modelling - Abstracts

ISBN

978-0-9565472-7-9

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

1

Strana od-do

31

Název nakladatele

University of Naples Federico II

Místo vydání

Naples

Místo konání akce

Naples

Datum konání akce

4. 9. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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