An hplc method for the differentiation of prenylated flavonoids from Paulownia tomentosa fruits

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

An hplc method for the differentiation of prenylated flavonoids from Paulownia tomentosa fruits

Popis výsledku v původním jazyce

Paulownia tomentosa (Thunb.) Siebold &amp; Zucc. ex Steud. (Paulowniaceae) is very adaptable and extremely fast growing timber tree native to central and western China traditionally used in Chinese medicine. It is a rich source of multifarious secondary metabolites, mainly prenylated flavonoids. Prenylated flavonoids are a large group of compounds that combine a flavonoid skeleton with a prenyl side-chain. Generally, the addition of an isoprenoid chain renders the derivate molecule more pharmacologically effective than the parent compound, probably because the prenyl group increases the lipophilicity and confers a strong affinity for biological membranes. Prenylated flavonoids exhibit potent antioxidant, antibacterial, cytotoxic and antiphlogistic activities. More than 50 compounds with modified prenyl or geranyl side-chains attached to the flavonoid skeleton have been isolated from the flowers, fruits, and leaves of P. tomentosa. Only two of these compounds have shown the presence of a five-carbon side-chain, for the others a ten-carbon side-chain is typical [1]. The goal of this contribution was to develop an HPLC method for the differentiation of 21 prenylated flavonoids previously isolated from P. tomentosa fruits. An HPLC method was modified for our purpose. The gradient elution used a mixture of acetonitril and water with 0.2% of formic acid. The gradient of the mobile phase initially consisted of 40% of acetonitril and reached 85% in the 25th minute. The analytes retention times were between 5 and 20 minutes. An HPLC method for the differentiation of 21 prenylated flavonoids from P. tomentosa fruits was developed and a library of these compounds was created. It can be used for the differentiation of these prenylflavonoids according to their retention time and UV spectra.

Název v anglickém jazyce

An hplc method for the differentiation of prenylated flavonoids from Paulownia tomentosa fruits

Popis výsledku anglicky

Paulownia tomentosa (Thunb.) Siebold &amp; Zucc. ex Steud. (Paulowniaceae) is very adaptable and extremely fast growing timber tree native to central and western China traditionally used in Chinese medicine. It is a rich source of multifarious secondary metabolites, mainly prenylated flavonoids. Prenylated flavonoids are a large group of compounds that combine a flavonoid skeleton with a prenyl side-chain. Generally, the addition of an isoprenoid chain renders the derivate molecule more pharmacologically effective than the parent compound, probably because the prenyl group increases the lipophilicity and confers a strong affinity for biological membranes. Prenylated flavonoids exhibit potent antioxidant, antibacterial, cytotoxic and antiphlogistic activities. More than 50 compounds with modified prenyl or geranyl side-chains attached to the flavonoid skeleton have been isolated from the flowers, fruits, and leaves of P. tomentosa. Only two of these compounds have shown the presence of a five-carbon side-chain, for the others a ten-carbon side-chain is typical [1]. The goal of this contribution was to develop an HPLC method for the differentiation of 21 prenylated flavonoids previously isolated from P. tomentosa fruits. An HPLC method was modified for our purpose. The gradient elution used a mixture of acetonitril and water with 0.2% of formic acid. The gradient of the mobile phase initially consisted of 40% of acetonitril and reached 85% in the 25th minute. The analytes retention times were between 5 and 20 minutes. An HPLC method for the differentiation of 21 prenylated flavonoids from P. tomentosa fruits was developed and a library of these compounds was created. It can be used for the differentiation of these prenylflavonoids according to their retention time and UV spectra.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10611 - Plant sciences, botany

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů