Curcumin encapsulation in yeast glucan particles promotes its anti-inflammatory potential in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F19%3A43877653" target="_blank" >RIV/62157124:16370/19:43877653 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/19:73596664 RIV/60461373:22340/19:43919141

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0378517319305769?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0378517319305769?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijpharm.2019.118532" target="_blank" >10.1016/j.ijpharm.2019.118532</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Curcumin encapsulation in yeast glucan particles promotes its anti-inflammatory potential in vitro

Popis výsledku v původním jazyce

Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of beta-1,3-D-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo. Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases. The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites. GP/curcumin composites were prepared using controlled evaporation of the organic solvent and their anti-oxidative effect and anti-inflammatory potential were tested on THP1-XBlue (TM)-MD2-CD14 human monocytes cell line. The anti-oxidative effect was measured on pyocyanin-stimulated cells in vitro and the NF-kappa B/AP-1 signaling pathway on lipopolysaccharide pre-treated monocytes was chosen for anti-inflammatory assays. The secretion of pro-inflammatory cytokines TNF-alpha and IL-1 beta was evaluated as well. Results mostly showed a pro-oxidative activity of empty GPs, however, pharmaceutical composites demonstrated an anti-oxidative effect. The activity of NF-kappa B/AP-1 was substantially decreased by the tested GP/curcumin composites, which also caused the attenuation of cytokines secretion. The obtained results indicate a beneficial effect of the incorporation of curcumin into GPs.

Název v anglickém jazyce

Curcumin encapsulation in yeast glucan particles promotes its anti-inflammatory potential in vitro

Popis výsledku anglicky

Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of beta-1,3-D-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo. Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases. The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites. GP/curcumin composites were prepared using controlled evaporation of the organic solvent and their anti-oxidative effect and anti-inflammatory potential were tested on THP1-XBlue (TM)-MD2-CD14 human monocytes cell line. The anti-oxidative effect was measured on pyocyanin-stimulated cells in vitro and the NF-kappa B/AP-1 signaling pathway on lipopolysaccharide pre-treated monocytes was chosen for anti-inflammatory assays. The secretion of pro-inflammatory cytokines TNF-alpha and IL-1 beta was evaluated as well. Results mostly showed a pro-oxidative activity of empty GPs, however, pharmaceutical composites demonstrated an anti-oxidative effect. The activity of NF-kappa B/AP-1 was substantially decreased by the tested GP/curcumin composites, which also caused the attenuation of cytokines secretion. The obtained results indicate a beneficial effect of the incorporation of curcumin into GPs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/NV16-27522A" target="_blank" >NV16-27522A: Přírodní bioaktivní látky enkapsulované v glukanových mikročásticích jako biomateriál vhodný pro léčbu střevních zánětlivých onemocnění</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Pharmaceutics

ISSN

0378-5173

e-ISSN

—

Svazek periodika

568

Číslo periodika v rámci svazku

568

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

—

Kód UT WoS článku

000482624300015

EID výsledku v databázi Scopus

2-s2.0-85069637773