Rapid HPLC method for monitoring of lactulose production with a high yield

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F19%3A43878174" target="_blank" >RIV/62157124:16370/19:43878174 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14160/19:00120952

Výsledek na webu

<a href="https://doi.org/10.1016/j.carres.2019.107773" target="_blank" >https://doi.org/10.1016/j.carres.2019.107773</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.carres.2019.107773" target="_blank" >10.1016/j.carres.2019.107773</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Rapid HPLC method for monitoring of lactulose production with a high yield

Popis výsledku v původním jazyce

An HPLC method suitable for rapid monitoring of lactulose production by isomerization from lactose was developed. The separation of lactose and lactulose under hydrophilic interaction liquid chromatography (HILIC) mode was achieved with resolution 1.5 within 5 min. Since isocratic elution was used, there is no extra time necessary for the column equilibration. Application of the method was illustrated on monitoring lactulose isomerization with catalysis of sodium hydroxide in the presence of sodium tetraborate at 70 degrees C (pH = 11). The conversion yield obtained for lactulose was 86%, and corresponding purity 76%. For the first time, a poly-hydroxy stationary phase for separation of lactose and lactulose is reported.

Název v anglickém jazyce

Rapid HPLC method for monitoring of lactulose production with a high yield

Popis výsledku anglicky

An HPLC method suitable for rapid monitoring of lactulose production by isomerization from lactose was developed. The separation of lactose and lactulose under hydrophilic interaction liquid chromatography (HILIC) mode was achieved with resolution 1.5 within 5 min. Since isocratic elution was used, there is no extra time necessary for the column equilibration. Application of the method was illustrated on monitoring lactulose isomerization with catalysis of sodium hydroxide in the presence of sodium tetraborate at 70 degrees C (pH = 11). The conversion yield obtained for lactulose was 86%, and corresponding purity 76%. For the first time, a poly-hydroxy stationary phase for separation of lactose and lactulose is reported.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Carbohydrate Research

ISSN

0008-6215

e-ISSN

—

Svazek periodika

484

Číslo periodika v rámci svazku

October

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

—

Kód UT WoS článku

000487202700004

EID výsledku v databázi Scopus

2-s2.0-85070197094