Plasmid-mediated resistance to colistin in healthy chicken in Paraguay

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Plasmid-mediated resistance to colistin in healthy chicken in Paraguay

Popis výsledku v původním jazyce

According to WHO, the antibiotic resistance is one of the major problems to deal with in our millennium. High occurrence of multi-drug resistant Gram-negative bacteria led to reintroducing less user-friendly antibiotics such as colistin to human medicine. Until the discovery of mcr-1 gene in 2016, only chromosomally-encoded resistance to colistin was observed. This type of resistance can be transferred only to other generations of bacteria. However, mcr (mobile colistin resistance) genes are plasmid-mediated and can be transferred very easily even within different Enterobacterales and cause problems in treatment of bacterial infections. We observed high prevalence (24%; 16/66) of mcr-5 gene in our samples from finisher broiler chicken from 12 different farms in Paraguay. We obtained 28 mcr-5-carrying Escherichia coli isolates and characterize them using diverse microbiological and molecular methods including whole-genome sequencing and conjugation experiments. We were the first to detect mcr-5 gene within conjugative plasmids of various types. This indicates it can be transfered more easily than it was originally thought as it was detected only within small non-conjugative plasmids before. Moreover, we observed frequent association of mcr-5 and genes encoding resistance to extendedspectrum beta-lactams in the same plasmid ? IncI1/ST113-mcr-5-blaCTX-M-8 and IncI1/ST12-mcr-5-blaCMY-2. IncI1/ST113 and IncI1/ST12 are epidemic plasmid lineages disseminating the beta-lactamase genes worldwide. Their association with mcr-5 represents a big threat for public health.

Název v anglickém jazyce

Plasmid-mediated resistance to colistin in healthy chicken in Paraguay

Popis výsledku anglicky

According to WHO, the antibiotic resistance is one of the major problems to deal with in our millennium. High occurrence of multi-drug resistant Gram-negative bacteria led to reintroducing less user-friendly antibiotics such as colistin to human medicine. Until the discovery of mcr-1 gene in 2016, only chromosomally-encoded resistance to colistin was observed. This type of resistance can be transferred only to other generations of bacteria. However, mcr (mobile colistin resistance) genes are plasmid-mediated and can be transferred very easily even within different Enterobacterales and cause problems in treatment of bacterial infections. We observed high prevalence (24%; 16/66) of mcr-5 gene in our samples from finisher broiler chicken from 12 different farms in Paraguay. We obtained 28 mcr-5-carrying Escherichia coli isolates and characterize them using diverse microbiological and molecular methods including whole-genome sequencing and conjugation experiments. We were the first to detect mcr-5 gene within conjugative plasmids of various types. This indicates it can be transfered more easily than it was originally thought as it was detected only within small non-conjugative plasmids before. Moreover, we observed frequent association of mcr-5 and genes encoding resistance to extendedspectrum beta-lactams in the same plasmid ? IncI1/ST113-mcr-5-blaCTX-M-8 and IncI1/ST12-mcr-5-blaCMY-2. IncI1/ST113 and IncI1/ST12 are epidemic plasmid lineages disseminating the beta-lactamase genes worldwide. Their association with mcr-5 represents a big threat for public health.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů