Rifampicin-loaded PLGA nanoparticles for local treatment of musculoskeletal infections: Formulation and characterization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F22%3A50019216" target="_blank" >RIV/62690094:18470/22:50019216 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/22:10444240 RIV/00216208:11160/22:10444240 RIV/00179906:_____/22:10444240

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1773224722003458" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1773224722003458</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jddst.2022.103435" target="_blank" >10.1016/j.jddst.2022.103435</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Rifampicin-loaded PLGA nanoparticles for local treatment of musculoskeletal infections: Formulation and characterization

Popis výsledku v původním jazyce

Systemic treatment of orthopedic device-related infections requires potentially toxic doses to reach local therapeutic levels. A locally applied nanosized drug delivery system can provide a high drug dose at the target site. Branched PLGA of lower molar mass was used for rifampicin-loaded nanoparticles (RIF-NPs) formulation by the nanoprecipitation method. The formulation variables influenced the NPs size ranging from 200 to 380 nm. The missing peak of crystalline rifampicin on the DSC scan of the NPs indicates that the drug was molecularly dispersed. The negative charge of the NPs in the physiological buffer was masked by a nonionic surfactant or converted to a positive one by a cationic surfactant, which promotes bioadhesion, penetration, and antimicrobial effect of locally applied RIF-NPs without being toxic to proliferation. Low values of loading capacity showed that PLGA predominates in the NPs, which, however, perform their function as a drug carrier ensuring sustained RIF release. The ex vivo dissolution study of RIF-NPs impregnated into the cancellous allogeneic bone grafts showed a low initial burst followed by sustained release of RIF for 7 days. Rifampicin-loaded PLGA nanoparticles may contribute to the prevention of resistant biofilm formation in patients with implanted devices and the local treatment of infections in open musculoskeletal injuries.

Název v anglickém jazyce

Rifampicin-loaded PLGA nanoparticles for local treatment of musculoskeletal infections: Formulation and characterization

Popis výsledku anglicky

Systemic treatment of orthopedic device-related infections requires potentially toxic doses to reach local therapeutic levels. A locally applied nanosized drug delivery system can provide a high drug dose at the target site. Branched PLGA of lower molar mass was used for rifampicin-loaded nanoparticles (RIF-NPs) formulation by the nanoprecipitation method. The formulation variables influenced the NPs size ranging from 200 to 380 nm. The missing peak of crystalline rifampicin on the DSC scan of the NPs indicates that the drug was molecularly dispersed. The negative charge of the NPs in the physiological buffer was masked by a nonionic surfactant or converted to a positive one by a cationic surfactant, which promotes bioadhesion, penetration, and antimicrobial effect of locally applied RIF-NPs without being toxic to proliferation. Low values of loading capacity showed that PLGA predominates in the NPs, which, however, perform their function as a drug carrier ensuring sustained RIF release. The ex vivo dissolution study of RIF-NPs impregnated into the cancellous allogeneic bone grafts showed a low initial burst followed by sustained release of RIF for 7 days. Rifampicin-loaded PLGA nanoparticles may contribute to the prevention of resistant biofilm formation in patients with implanted devices and the local treatment of infections in open musculoskeletal injuries.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY

ISSN

1773-2247

e-ISSN

2588-8943

Svazek periodika

73

Číslo periodika v rámci svazku

JUL 2022

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

"Article Number: 103435"

Kód UT WoS článku

000806232000005

EID výsledku v databázi Scopus

2-s2.0-85130230828