Multiple productive IGH rearrangements denote oligoclonality even in immunophenotypically monoclonal CLL

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F18%3A00068192" target="_blank" >RIV/65269705:_____/18:00068192 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/18:00102140

Výsledek na webu

<a href="http://www.nature.com/articles/leu2017274" target="_blank" >http://www.nature.com/articles/leu2017274</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/leu.2017.274" target="_blank" >10.1038/leu.2017.274</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multiple productive IGH rearrangements denote oligoclonality even in immunophenotypically monoclonal CLL

Popis výsledku v původním jazyce

In our systematic study of CLL cases with multiple productive IGH rearrangements (MP-IGH CLL),3 we previously showed that one-third of them exhibit two CLL populations with distinct immunophenotypes. We also pointed to the presence of multiple clones as a likely cause of MP-IGH detection even in cases with an immunophenotypically monoclonal disease, on the basis of IGK, IGL and incomplete IGH gene rearrangement analyses. Still, the underlying biological cause of MP-IGHs possibly involves lack of allelic exclusion4 or IGHV gene replacement.5 The extent to which these mechanisms are involved in MP-IGH CLL cases with homogeneous immunophenotype is still not known, and the lack of conclusive evidence hinders further research on CLL oligoclonality. To clarify this phenomenon, we performed extensive analysis of clonality in MP-IGH CLL combining conventional methods (Sanger sequencing, IGH fragment analysis), immunophenotyping, single-cell analysis (SCA), and next-generation sequencing (NGS).

Název v anglickém jazyce

Multiple productive IGH rearrangements denote oligoclonality even in immunophenotypically monoclonal CLL

Popis výsledku anglicky

In our systematic study of CLL cases with multiple productive IGH rearrangements (MP-IGH CLL),3 we previously showed that one-third of them exhibit two CLL populations with distinct immunophenotypes. We also pointed to the presence of multiple clones as a likely cause of MP-IGH detection even in cases with an immunophenotypically monoclonal disease, on the basis of IGK, IGL and incomplete IGH gene rearrangement analyses. Still, the underlying biological cause of MP-IGHs possibly involves lack of allelic exclusion4 or IGHV gene replacement.5 The extent to which these mechanisms are involved in MP-IGH CLL cases with homogeneous immunophenotype is still not known, and the lack of conclusive evidence hinders further research on CLL oligoclonality. To clarify this phenomenon, we performed extensive analysis of clonality in MP-IGH CLL combining conventional methods (Sanger sequencing, IGH fragment analysis), immunophenotyping, single-cell analysis (SCA), and next-generation sequencing (NGS).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30204 - Oncology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Leukemia

ISSN

0887-6924

e-ISSN

—

Svazek periodika

32

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

3

Strana od-do

234-236

Kód UT WoS článku

000419771800027

EID výsledku v databázi Scopus

—