Tracking minor clones in chronic lymphocytic leukemia using high-throughput immunoprofiling

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F18%3A00068760" target="_blank" >RIV/65269705:_____/18:00068760 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/18:00103335

Výsledek na webu

<a href="http://phdretreat.ceitec.cz/ceitec-phd-and-postdoc-retreat-2018/" target="_blank" >http://phdretreat.ceitec.cz/ceitec-phd-and-postdoc-retreat-2018/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Tracking minor clones in chronic lymphocytic leukemia using high-throughput immunoprofiling

Popis výsledku v původním jazyce

In chronic lymphocytic leukemia (CLL), multiple clones can be detected in up to a quar-ter of cases. Typically, a major clone coexists with minor one(s). Shifts in clonal ratios and prevailing of initially minor clones in the course of the disease have been reported, therefore, their monitoring is desirable. We aimed to test the capability of NGS im-munoprofiling methods to detect minor clones in oligoclonal CLL. We focused primar-ily on cases showing result discrepancies using various methods, and cases with con-siderable clonal shifts during disease course. Overall, 21 samples from 14 patients (7 patients were monitored in several time-points) were submitted to NGS immunoprofiling with DNA and RNA as a starting material. The results from both methods were compared to those obtained using bulk sample Sanger sequencing and single cell analysis. We report that detection of minor clones in oligoclonal CLL is challenging, since its efficiency varies with different approaches. As the clinic-biological significance of minor clones in general is still unclear, it warrants further detailed investigation in a larger cohort.

Název v anglickém jazyce

Tracking minor clones in chronic lymphocytic leukemia using high-throughput immunoprofiling

Popis výsledku anglicky

In chronic lymphocytic leukemia (CLL), multiple clones can be detected in up to a quar-ter of cases. Typically, a major clone coexists with minor one(s). Shifts in clonal ratios and prevailing of initially minor clones in the course of the disease have been reported, therefore, their monitoring is desirable. We aimed to test the capability of NGS im-munoprofiling methods to detect minor clones in oligoclonal CLL. We focused primar-ily on cases showing result discrepancies using various methods, and cases with con-siderable clonal shifts during disease course. Overall, 21 samples from 14 patients (7 patients were monitored in several time-points) were submitted to NGS immunoprofiling with DNA and RNA as a starting material. The results from both methods were compared to those obtained using bulk sample Sanger sequencing and single cell analysis. We report that detection of minor clones in oligoclonal CLL is challenging, since its efficiency varies with different approaches. As the clinic-biological significance of minor clones in general is still unclear, it warrants further detailed investigation in a larger cohort.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NV15-30015A" target="_blank" >NV15-30015A: Analýza klonální heterogenity chronické lymfocytární leukemie pomoci sekvenování nové generace genu pro B-buněčný receptor. Národní studie.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů