Capture-based NGS panel for detection of immunoglobulin and T-cell receptor gene rearrangements in DNA samples from patients with acute lymphoblastic leukemia
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F20%3A00073230" target="_blank" >RIV/65269705:_____/20:00073230 - isvavai.cz</a>
Výsledek na webu
<a href="https://journals.lww.com/hemasphere/Citation/2020/06001/Abstract_Book__25th_Congress_of_the_European.1.aspx" target="_blank" >https://journals.lww.com/hemasphere/Citation/2020/06001/Abstract_Book__25th_Congress_of_the_European.1.aspx</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1097/HS9.0000000000000404" target="_blank" >10.1097/HS9.0000000000000404</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Capture-based NGS panel for detection of immunoglobulin and T-cell receptor gene rearrangements in DNA samples from patients with acute lymphoblastic leukemia
Popis výsledku v původním jazyce
Background: Monitoring of minimal residual disease (MRD) is a strong prognostic factor of clinical outcome in ALL patients. There are several different techniques available for MRD detection. Real-time quantitative PCR (RT-qPCR) with patient-specific primers represents a method well standardized by EuroMRD Working Group, which is still widely used despite great advances in next-generation sequencing. Acquiring of individual immunoglobulin (IG)/T-cell receptor (TR) junctional region sequences for primer design using traditional Sanger sequencing is often difficult due to the presence of polyclonal background and due to the necessity of multiplex PCR usage for IG/TR gene amplification. Aims: Our aim was to assess applicability of our recently established versatile NGS panel for characterization of clonal IG/TR rearrangements in ALL samples.
Název v anglickém jazyce
Capture-based NGS panel for detection of immunoglobulin and T-cell receptor gene rearrangements in DNA samples from patients with acute lymphoblastic leukemia
Popis výsledku anglicky
Background: Monitoring of minimal residual disease (MRD) is a strong prognostic factor of clinical outcome in ALL patients. There are several different techniques available for MRD detection. Real-time quantitative PCR (RT-qPCR) with patient-specific primers represents a method well standardized by EuroMRD Working Group, which is still widely used despite great advances in next-generation sequencing. Acquiring of individual immunoglobulin (IG)/T-cell receptor (TR) junctional region sequences for primer design using traditional Sanger sequencing is often difficult due to the presence of polyclonal background and due to the necessity of multiplex PCR usage for IG/TR gene amplification. Aims: Our aim was to assess applicability of our recently established versatile NGS panel for characterization of clonal IG/TR rearrangements in ALL samples.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
—
OECD FORD obor
30205 - Hematology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů