Expression of genes involved in the inflammatory response in human granulosa cells in short-term in vitro culture

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F20%3A00074020" target="_blank" >RIV/65269705:_____/20:00074020 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciendo.com/article/10.2478/acb-2020-0025" target="_blank" >https://www.sciendo.com/article/10.2478/acb-2020-0025</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/acb-2020-0025" target="_blank" >10.2478/acb-2020-0025</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Expression of genes involved in the inflammatory response in human granulosa cells in short-term in vitro culture

Popis výsledku v původním jazyce

The essential function of granulosa cells is to maintain the proper course of oogenesis and folliculogenesis. The immune system is an additional local regulator of ovarian function, with cytokines necessary for the proper function of the ovaries, including the secretion of steroid hormones This study aimed to analyze the expression of genes in human GCs in short-term primary culture and define the difference in the expression of IL1β, IL6, and TNFα genes at 48h and 72h of culture compared to the 24h control. Total RNA was isolated using the Chomczyński and Sacchi protocol. RNA samples were treated with DNase I and reverse transcribed (RT) into cDNA. The determination of transcript levels of the mentioned genes was performed using the Light Cycler(R) 96 Real-Time PCR kit, Roche Diagnostics GmbH (Mannheim, Germany). The present study proved that granulosa cells in a short-term primary in vitro culture express IL-1β, IL-6, and TNFα. The tested genes show a decrease in expression at 24h of culture and a subsequent slight increase at 72h, not exceeding the initial levels. The expression changes the most for IL1β and the least for TNFα. The fluctuations in the amount of transcript may be influenced by factors stored in granulosa cells before the IVM procedure, the procedure of in vitro fertilization, as well as factors related to the process of primary culture. More research is needed to understand the details of these occurrences. Running title: The inflammatory response in human granulosa cells.

Název v anglickém jazyce

Expression of genes involved in the inflammatory response in human granulosa cells in short-term in vitro culture

Popis výsledku anglicky

The essential function of granulosa cells is to maintain the proper course of oogenesis and folliculogenesis. The immune system is an additional local regulator of ovarian function, with cytokines necessary for the proper function of the ovaries, including the secretion of steroid hormones This study aimed to analyze the expression of genes in human GCs in short-term primary culture and define the difference in the expression of IL1β, IL6, and TNFα genes at 48h and 72h of culture compared to the 24h control. Total RNA was isolated using the Chomczyński and Sacchi protocol. RNA samples were treated with DNase I and reverse transcribed (RT) into cDNA. The determination of transcript levels of the mentioned genes was performed using the Light Cycler(R) 96 Real-Time PCR kit, Roche Diagnostics GmbH (Mannheim, Germany). The present study proved that granulosa cells in a short-term primary in vitro culture express IL-1β, IL-6, and TNFα. The tested genes show a decrease in expression at 24h of culture and a subsequent slight increase at 72h, not exceeding the initial levels. The expression changes the most for IL1β and the least for TNFα. The fluctuations in the amount of transcript may be influenced by factors stored in granulosa cells before the IVM procedure, the procedure of in vitro fertilization, as well as factors related to the process of primary culture. More research is needed to understand the details of these occurrences. Running title: The inflammatory response in human granulosa cells.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Medical Journal of Cell Biology

ISSN

2544-3577

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

6

Strana od-do

190-195

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85100169897