Immunomagnetic separation of Escherichia coli O26, O111 and O157 from vegetables.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67179843%3A_____%2F01%3A22013036" target="_blank" >RIV/67179843:_____/01:22013036 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Immunomagnetic separation of Escherichia coli O26, O111 and O157 from vegetables.

Popis výsledku v původním jazyce

Raw vegetable and fruit products have been increasingly connected with human infections with Shiga toxin-producing Escherichia coli. This study evaluated the isolation and detection of E. coli O26, O111 and O157 inoculated into vegetable samples using immunomagnetic particles. Standard cultivation and immunomagnetic separation (IMS) procedures were compared. It was found that immunomagnetic particles very efficiently concentrate E. coli cells, detecting significantly more bacteria then with standard cultivation procedures. E. coli O111and O157 were detected in 93-100 % of inoculated samples using IMS, but only 36-53 % samples after plating were found positive. In the case of E. coli O26, 93 % samples after direct plating were positive compared with 100% positive samples after IMS; the number of presumptive colonies was significantly higher after IMS.

Název v anglickém jazyce

Immunomagnetic separation of Escherichia coli O26, O111 and O157 from vegetables.

Popis výsledku anglicky

Raw vegetable and fruit products have been increasingly connected with human infections with Shiga toxin-producing Escherichia coli. This study evaluated the isolation and detection of E. coli O26, O111 and O157 inoculated into vegetable samples using immunomagnetic particles. Standard cultivation and immunomagnetic separation (IMS) procedures were compared. It was found that immunomagnetic particles very efficiently concentrate E. coli cells, detecting significantly more bacteria then with standard cultivation procedures. E. coli O111and O157 were detected in 93-100 % of inoculated samples using IMS, but only 36-53 % samples after plating were found positive. In the case of E. coli O26, 93 % samples after direct plating were positive compared with 100% positive samples after IMS; the number of presumptive colonies was significantly higher after IMS.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F98%2F1145" target="_blank" >GA203/98/1145: Vývoj magnetických separačních technik pro isolaci biologicky aktivních látek</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Letters in Applied Microbiology

ISSN

0266-8254

e-ISSN

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Svazek periodika

33

Číslo periodika v rámci svazku

N/A

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

4

Strana od-do

36-39

Kód UT WoS článku

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EID výsledku v databázi Scopus

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