Immobilization of Recombinant CGTase JCGT8-5 on Magnetically-Modified Silicates and Natural Supports

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Immobilization of Recombinant CGTase JCGT8-5 on Magnetically-Modified Silicates and Natural Supports

Popis výsledku v původním jazyce

Recombinant cyclodextrin glucanotransferase JCGT8-5 (CGTase, EC 2.4.1.19) was effectively immobilized by adsorption on magnetically-modified particles. Silicates (montmorillonite and halloysite), natural supports (oat straw, wheat bran, banana peel, sawdust, hazelnut and peanut shell, coffee beans, tea leaves, algae Chlorella vulgaris) and activated carbon were engaged as carriers, as the most of them were used for the first time for enzyme adsorption. The immobilization capacities of the carriers entrapping ultra-concentrate or purified enzyme were determined. Binding yields reached from 50% to 90% of the initial enzyme quantity. The analysis of the formed cyclodextrins (CDs) revealed that the highest amount of total CDs was obtained by the use of halloysite particles (10.1 mg/ml), followed by sawdust (8.6 mg/ml), algae (8.6 mg/ml) and peanut shell (8.2 mg/ml). A high degree of starch conversion into CDs, ranging from 21 to 25% was achieved for 20 min starch hydrolysis. Variations in

Název v anglickém jazyce

Immobilization of Recombinant CGTase JCGT8-5 on Magnetically-Modified Silicates and Natural Supports

Popis výsledku anglicky

Recombinant cyclodextrin glucanotransferase JCGT8-5 (CGTase, EC 2.4.1.19) was effectively immobilized by adsorption on magnetically-modified particles. Silicates (montmorillonite and halloysite), natural supports (oat straw, wheat bran, banana peel, sawdust, hazelnut and peanut shell, coffee beans, tea leaves, algae Chlorella vulgaris) and activated carbon were engaged as carriers, as the most of them were used for the first time for enzyme adsorption. The immobilization capacities of the carriers entrapping ultra-concentrate or purified enzyme were determined. Binding yields reached from 50% to 90% of the initial enzyme quantity. The analysis of the formed cyclodextrins (CDs) revealed that the highest amount of total CDs was obtained by the use of halloysite particles (10.1 mg/ml), followed by sawdust (8.6 mg/ml), algae (8.6 mg/ml) and peanut shell (8.2 mg/ml). A high degree of starch conversion into CDs, ranging from 21 to 25% was achieved for 20 min starch hydrolysis. Variations in

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Review of Chemical Engineering

ISSN

2035-1755

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

BG - Bulharská republika

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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