Optimalization of production of cyclodextrin glycosyl transferase by Paenibacillus macerans strain and cyclodextrin formation (Abstract: Proceedings of The 5th Asian Cyclodextrin Conference (ACC2009). May 13-16, 2009. Busan, Korea)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F09%3A00000129" target="_blank" >RIV/00027022:_____/09:00000129 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Optimalization of production of cyclodextrin glycosyl transferase by Paenibacillus macerans strain and cyclodextrin formation (Abstract: Proceedings of The 5th Asian Cyclodextrin Conference (ACC2009). May 13-16, 2009. Busan, Korea)

Popis výsledku v původním jazyce

Cyclodextrins (CDs) are synthesized by the bacterial extracellular enzym cyclodextrin glycosyltransferase (CGTase, E.C. 2.4.1.19) from starch or starch derivatives via an inter- and intramolecural transglycosylation reaction. The production of ?- CD, ß-CD and ?- CD by CGTase from Paenibacillus macerans was studied, in regard to the effect of the source starch (amaranth, corn) on the yield of CDs. The CGTase was produced by a 3-day sterile cultivation in the laboratory Bench-top fermentor BiostatB in acomplex medium containing starch and partially purified by ammonium sulphate precipitation at 60% saturation. Apparent molecular weight as determined by SDS?PAGE was in the range from 105 to 114 kDa. All the commercially important ?-, ß-, and ?-CDs weredetected chromatographically after hydrolysis of the corn and amaranth (Amaranthus cruentus) starch with the isolated enzyme.

Název v anglickém jazyce

Optimalization of production of cyclodextrin glycosyl transferase by Paenibacillus macerans strain and cyclodextrin formation (Abstract: Proceedings of The 5th Asian Cyclodextrin Conference (ACC2009). May 13-16, 2009. Busan, Korea)

Popis výsledku anglicky

Cyclodextrins (CDs) are synthesized by the bacterial extracellular enzym cyclodextrin glycosyltransferase (CGTase, E.C. 2.4.1.19) from starch or starch derivatives via an inter- and intramolecural transglycosylation reaction. The production of ?- CD, ß-CD and ?- CD by CGTase from Paenibacillus macerans was studied, in regard to the effect of the source starch (amaranth, corn) on the yield of CDs. The CGTase was produced by a 3-day sterile cultivation in the laboratory Bench-top fermentor BiostatB in acomplex medium containing starch and partially purified by ammonium sulphate precipitation at 60% saturation. Apparent molecular weight as determined by SDS?PAGE was in the range from 105 to 114 kDa. All the commercially important ?-, ß-, and ?-CDs weredetected chromatographically after hydrolysis of the corn and amaranth (Amaranthus cruentus) starch with the isolated enzyme.

Druh

O - Ostatní výsledky

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/2B06173" target="_blank" >2B06173: Materiály a produkty s vysokou přidanou hodnotou ze zemědělských a potravinářských odpadů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů