Cyclodextrin production from an amaranth starch by cyclodextrin glycosyltransferase produced by Paenibacillus macerans CCM 2012

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F12%3A00000337" target="_blank" >RIV/00027022:_____/12:00000337 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Cyclodextrin production from an amaranth starch by cyclodextrin glycosyltransferase produced by Paenibacillus macerans CCM 2012

Popis výsledku v původním jazyce

Cyclodextrins (CDs) are synthesised by bacterial extracellular enzym cyclodextrin glycosyltransferase (CGTase, E.C. 2.4.1.19) from starch or starch derivatives. The production of ?-, ß-, and ?-CDs by CGTase from Paenibacillus macerans CCM 2012 was studied in regard to the effect of the starch source (amaranth, maize) on the yield of CDs. CGTase was produced by a 3-day sterile cultivation in the laboratory Bench-top fermentor BiostatB under aerobic conditions. CGTase was partially purified by ammonium sulfate precipitation at 60% saturation. Electrophoretic analysis (SDS-PAGE) of the isolated CGTase enzyme was carried out according to the method by Laemmli (1970), the apparent molecular weight was in the range from 105 kDa to 114 kDa. All the commercially important ?-, ß-, and ?-CDs were detected chromatographically after the hydrolysis of the maize and amaranth (amaranthus cruentus) starches with the isolated enzyme. The amaranth starch appears to be an excellent substrate for CDs prod

Název v anglickém jazyce

Cyclodextrin production from an amaranth starch by cyclodextrin glycosyltransferase produced by Paenibacillus macerans CCM 2012

Popis výsledku anglicky

Cyclodextrins (CDs) are synthesised by bacterial extracellular enzym cyclodextrin glycosyltransferase (CGTase, E.C. 2.4.1.19) from starch or starch derivatives. The production of ?-, ß-, and ?-CDs by CGTase from Paenibacillus macerans CCM 2012 was studied in regard to the effect of the starch source (amaranth, maize) on the yield of CDs. CGTase was produced by a 3-day sterile cultivation in the laboratory Bench-top fermentor BiostatB under aerobic conditions. CGTase was partially purified by ammonium sulfate precipitation at 60% saturation. Electrophoretic analysis (SDS-PAGE) of the isolated CGTase enzyme was carried out according to the method by Laemmli (1970), the apparent molecular weight was in the range from 105 kDa to 114 kDa. All the commercially important ?-, ß-, and ?-CDs were detected chromatographically after the hydrolysis of the maize and amaranth (amaranthus cruentus) starches with the isolated enzyme. The amaranth starch appears to be an excellent substrate for CDs prod

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/2B06173" target="_blank" >2B06173: Materiály a produkty s vysokou přidanou hodnotou ze zemědělských a potravinářských odpadů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Food Sciences

ISSN

1212-1800

e-ISSN

—

Svazek periodika

30

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

15-20

Kód UT WoS článku

000300598500003

EID výsledku v databázi Scopus

—