Volume Reconstruction Using Confocal Microscopy Images and Correction of Deformations by Elastic Registration

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F09%3A00329247" target="_blank" >RIV/67985823:_____/09:00329247 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21460/09:00156738

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Volume Reconstruction Using Confocal Microscopy Images and Correction of Deformations by Elastic Registration

Popis výsledku v původním jazyce

A set of methods leading to volume reconstruction of biological specimens larger than the field of view of a confocal laser scanning microscope (CLSM) is presented. Large tissue specimens are cut into thin physical slices and volume data sets are captured from all studied physical slices by CLSM. Overlapping spatial tiles of the same physical slice are stitched in horizontal direction. Image volumes of successive physical slices are linked in axial direction by applying an elastic registration algorithmto compensate for deformations due to cutting the specimen. We present a method enabling us to keep true object morphology using a-priori information about the shape and size of the specimen, available from images of the cutting planes captured by a USBlight microscope immediately before cutting the specimen by a microtome. The errors introduced by elastic registration are evaluated using a stereological point counting method and the Procrustes distance

Název v anglickém jazyce

Volume Reconstruction Using Confocal Microscopy Images and Correction of Deformations by Elastic Registration

Popis výsledku anglicky

A set of methods leading to volume reconstruction of biological specimens larger than the field of view of a confocal laser scanning microscope (CLSM) is presented. Large tissue specimens are cut into thin physical slices and volume data sets are captured from all studied physical slices by CLSM. Overlapping spatial tiles of the same physical slice are stitched in horizontal direction. Image volumes of successive physical slices are linked in axial direction by applying an elastic registration algorithmto compensate for deformations due to cutting the specimen. We present a method enabling us to keep true object morphology using a-priori information about the shape and size of the specimen, available from images of the cutting planes captured by a USBlight microscope immediately before cutting the specimen by a microtome. The errors introduced by elastic registration are evaluated using a stereological point counting method and the Procrustes distance

Druh

D - Stať ve sborníku

CEP obor

JC - Počítačový hardware a software

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Stereology and Image Analysis

ISBN

978-88-7488-310-3

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

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Název nakladatele

Esculapio

Místo vydání

Bologna

Místo konání akce

Milano

Datum konání akce

22. 6. 2009

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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