Proteomic analysis of human tooth pulp proteomes – Comparison of caries-resistant and caries-susceptible

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F16%3A00462855" target="_blank" >RIV/67985823:_____/16:00462855 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jprot.2016.04.022" target="_blank" >http://dx.doi.org/10.1016/j.jprot.2016.04.022</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jprot.2016.04.022" target="_blank" >10.1016/j.jprot.2016.04.022</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Proteomic analysis of human tooth pulp proteomes – Comparison of caries-resistant and caries-susceptible

Popis výsledku v původním jazyce

Most people in the world suffer from dental caries, > 90% of adults experience caries on enamel and root surfaces during their life. However, the overall roles of all factors in the development of dental caries still remain unclear and are worthy of recent investigation. In this study we used a proteomic 2D-DIGE approach in connection with MS/MS to investigate the different protein abundances in the tooth pulp of human third molars obtained from caries-resistant and caries-susceptible people. Statistical analysis of the two protein maps obtained on large gel (17 cm length) and mini gel (7 cm length) followed by nLC-MS/MS analysis enabled the identification of 16 significantly changed spots with unique protein identifications corresponding to 12 non-redundant proteins. Seven proteins exhibited higher and four proteins exhibited lower expression in the caries-resistant samples compared to the caries-susceptible samples. Additionally, one protein (alpha-1-antitrypsin) exhibited both expressions (up and down). Most of the differentially expressed proteins were associated with protein metabolism, energy production, cytoskeletal organization and transport. These differentially expressed proteins are likely involved in the natural resistance or susceptibility of humans to the development of dental caries and suggest that the resistance mechanism is multifactorial.

Název v anglickém jazyce

Proteomic analysis of human tooth pulp proteomes – Comparison of caries-resistant and caries-susceptible

Popis výsledku anglicky

Most people in the world suffer from dental caries, > 90% of adults experience caries on enamel and root surfaces during their life. However, the overall roles of all factors in the development of dental caries still remain unclear and are worthy of recent investigation. In this study we used a proteomic 2D-DIGE approach in connection with MS/MS to investigate the different protein abundances in the tooth pulp of human third molars obtained from caries-resistant and caries-susceptible people. Statistical analysis of the two protein maps obtained on large gel (17 cm length) and mini gel (7 cm length) followed by nLC-MS/MS analysis enabled the identification of 16 significantly changed spots with unique protein identifications corresponding to 12 non-redundant proteins. Seven proteins exhibited higher and four proteins exhibited lower expression in the caries-resistant samples compared to the caries-susceptible samples. Additionally, one protein (alpha-1-antitrypsin) exhibited both expressions (up and down). Most of the differentially expressed proteins were associated with protein metabolism, energy production, cytoskeletal organization and transport. These differentially expressed proteins are likely involved in the natural resistance or susceptibility of humans to the development of dental caries and suggest that the resistance mechanism is multifactorial.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FF - ORL, oftalmologie, stomatologie

OECD FORD obor

—

Projekt

<a href="/cs/project/NT14324" target="_blank" >NT14324: Srovnávací studie proteinového složení slin, pelikuly a zubů ve vztahu k zubnímu kazu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Proteomics

ISSN

1874-3919

e-ISSN

—

Svazek periodika

145

Číslo periodika v rámci svazku

Aug 11

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

127-136

Kód UT WoS článku

000383931000013

EID výsledku v databázi Scopus

2-s2.0-84964864688