Chronic intermittent hypoxia affects the cytosolic phospholipase A(2)alpha/cyclooxygenase 2 pathway via beta(2)-adrenoceptor-mediated ERK/p38 stimulation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F16%3A00466474" target="_blank" >RIV/67985823:_____/16:00466474 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/16:10332874

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11010-016-2833-8" target="_blank" >http://dx.doi.org/10.1007/s11010-016-2833-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11010-016-2833-8" target="_blank" >10.1007/s11010-016-2833-8</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chronic intermittent hypoxia affects the cytosolic phospholipase A(2)alpha/cyclooxygenase 2 pathway via beta(2)-adrenoceptor-mediated ERK/p38 stimulation

Popis výsledku v původním jazyce

Cardiac resistance against acute ischemia/reperfusion (I/R) injury can be enhanced by adaptation to chronic intermittent hypoxia (CIH), but the changes at the molecular level associated with this adaptation are still not fully explored. Phospholipase A(2) (PLA(2)) plays an important role in phospholipid metabolism and may contribute to membrane destruction under conditions of energy deprivation during I/R. The aim of this study was to determine the effect of CIH (7000 m, 8 h/day, 5 weeks) on the expression of cytosolic PLA(2)alpha (cPLA(2)alpha) and its phosphorylated form (p-cPLA(2)alpha), as well as other related signaling proteins in the left ventricular myocardium of adult male Wistar rats. Adaptation to CIH increased the total content of cPLA(2)alpha by 14 % in myocardial homogenate, and enhanced the association of p-cPLA(2)alpha with the nuclear membrane by 85 %. The total number of beta-adrenoceptors (beta-ARs) did not change but the beta(2)/beta(1) ratio markedly increased due to the elevation of beta(2)-ARs and drop in beta(1)-ARs. In parallel, the amount of adenylyl cyclase decreased by 49 % and G(i)alpha proteins increased by about 50 %. Besides that, cyclooxygenase 2 (COX-2) and prostaglandin E-2 (PGE(2)) increased by 36 and 84 %, respectively. In parallel, we detected increased phosphorylation of protein kinase C alpha, ERK1/2 and p38 (by 12, 48 and 19 %, respectively). These data suggest that adaptive changes induced in the myocardium by CIH may include activation of cPLA(2)alpha and COX-2 via beta(2)-AR/G(i)-mediated stimulation of the ERK/p38 pathway.

Název v anglickém jazyce

Chronic intermittent hypoxia affects the cytosolic phospholipase A(2)alpha/cyclooxygenase 2 pathway via beta(2)-adrenoceptor-mediated ERK/p38 stimulation

Popis výsledku anglicky

Cardiac resistance against acute ischemia/reperfusion (I/R) injury can be enhanced by adaptation to chronic intermittent hypoxia (CIH), but the changes at the molecular level associated with this adaptation are still not fully explored. Phospholipase A(2) (PLA(2)) plays an important role in phospholipid metabolism and may contribute to membrane destruction under conditions of energy deprivation during I/R. The aim of this study was to determine the effect of CIH (7000 m, 8 h/day, 5 weeks) on the expression of cytosolic PLA(2)alpha (cPLA(2)alpha) and its phosphorylated form (p-cPLA(2)alpha), as well as other related signaling proteins in the left ventricular myocardium of adult male Wistar rats. Adaptation to CIH increased the total content of cPLA(2)alpha by 14 % in myocardial homogenate, and enhanced the association of p-cPLA(2)alpha with the nuclear membrane by 85 %. The total number of beta-adrenoceptors (beta-ARs) did not change but the beta(2)/beta(1) ratio markedly increased due to the elevation of beta(2)-ARs and drop in beta(1)-ARs. In parallel, the amount of adenylyl cyclase decreased by 49 % and G(i)alpha proteins increased by about 50 %. Besides that, cyclooxygenase 2 (COX-2) and prostaglandin E-2 (PGE(2)) increased by 36 and 84 %, respectively. In parallel, we detected increased phosphorylation of protein kinase C alpha, ERK1/2 and p38 (by 12, 48 and 19 %, respectively). These data suggest that adaptive changes induced in the myocardium by CIH may include activation of cPLA(2)alpha and COX-2 via beta(2)-AR/G(i)-mediated stimulation of the ERK/p38 pathway.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FA - Kardiovaskulární nemoci včetně kardiochirurgie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA13-10267S" target="_blank" >GA13-10267S: Ischemická tolerance srdcí spontánně hypertenzních potkanů: význam mitochondriálního genomu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Cellular Biochemistry

ISSN

0300-8177

e-ISSN

—

Svazek periodika

423

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

151-163

Kód UT WoS článku

000387365600015

EID výsledku v databázi Scopus

2-s2.0-84989184220