Diversity in Seed Storage Protein Profile of Oilseed Crop Plukenetia volubilis from Peruvian Amazon

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00504502" target="_blank" >RIV/67985823:_____/19:00504502 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41340/19:79360 RIV/00027006:_____/19:00005353

Výsledek na webu

<a href="http://www.fspublishers.org/published_papers/23739_26%20doi%2015.0945%20IJAB-18-1080%20(10)%20679-688.pdf" target="_blank" >http://www.fspublishers.org/published_papers/23739_26%20doi%2015.0945%20IJAB-18-1080%20(10)%20679-688.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Diversity in Seed Storage Protein Profile of Oilseed Crop Plukenetia volubilis from Peruvian Amazon

Popis výsledku v původním jazyce

Sacha inchi (Plukenetia volubilis L.) is a plant native to the Peruvian Amazon and produces seeds rich in some nutraceutical compounds with a high protein content. The present work aimed to characterize P. volubilis seed proteins and compare their proteomic profile between different P. volubilis populations. Crude protein content in the seeds was detected at a mean level of 22.56% with the major proportion of albumins and globulins (16.37%), followed by glutelins (5.87%) and a very low content of prolamins (0.33%). Sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gel electrophoresis of both total seed flour and Osborne's protein fractions of different populations showed that proteins were concentrated in the 8-75 kDa range. Differences in abundance of proteins between Sacha inchi populations were detected by SD S-PAGE and two-dimensional (2-DE) gel electrophoresis. Gel protein bands and spots of interest were excised, digested with trypsin and analyzed by nano-liquid chromatography/tandem mass spectrometry (nLC-MS/MS). This is the first direct identification of these proteins in actual Sacha inchi seeds. Three proteins (Oleosin 2, Oleosin 3 and elongation factor 1-alpha) directly from P. volubilis and peptides with a sequence identical to 56 proteins from the Euphothiaceae family were detected. This characterization of seed storage protein, their variability within populations and identification of important proteins provide a basis for further investigations for the food industry and bioengineering.

Název v anglickém jazyce

Diversity in Seed Storage Protein Profile of Oilseed Crop Plukenetia volubilis from Peruvian Amazon

Popis výsledku anglicky

Sacha inchi (Plukenetia volubilis L.) is a plant native to the Peruvian Amazon and produces seeds rich in some nutraceutical compounds with a high protein content. The present work aimed to characterize P. volubilis seed proteins and compare their proteomic profile between different P. volubilis populations. Crude protein content in the seeds was detected at a mean level of 22.56% with the major proportion of albumins and globulins (16.37%), followed by glutelins (5.87%) and a very low content of prolamins (0.33%). Sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gel electrophoresis of both total seed flour and Osborne's protein fractions of different populations showed that proteins were concentrated in the 8-75 kDa range. Differences in abundance of proteins between Sacha inchi populations were detected by SD S-PAGE and two-dimensional (2-DE) gel electrophoresis. Gel protein bands and spots of interest were excised, digested with trypsin and analyzed by nano-liquid chromatography/tandem mass spectrometry (nLC-MS/MS). This is the first direct identification of these proteins in actual Sacha inchi seeds. Three proteins (Oleosin 2, Oleosin 3 and elongation factor 1-alpha) directly from P. volubilis and peptides with a sequence identical to 56 proteins from the Euphothiaceae family were detected. This characterization of seed storage protein, their variability within populations and identification of important proteins provide a basis for further investigations for the food industry and bioengineering.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA15-01948S" target="_blank" >GA15-01948S: Kapilární elektromigrační techniky využívající afinitní selektory a smart-polymery pro analýzu a studium vlastností a interakcí biomolekul.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Agriculture and Biology

ISSN

1560-8530

e-ISSN

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Svazek periodika

21

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

PK - Pákistánská islámská republika

Počet stran výsledku

10

Strana od-do

679-688

Kód UT WoS článku

000456544000026

EID výsledku v databázi Scopus

2-s2.0-85062499141