Participation of opioid receptors in the cytoprotective effect of chronic normobaric hypoxia

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00504694" target="_blank" >RIV/67985823:_____/19:00504694 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.biomed.cas.cz/physiolres/pdf/2019/68_245.pdf" target="_blank" >http://www.biomed.cas.cz/physiolres/pdf/2019/68_245.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.33549/physiolres.933938" target="_blank" >10.33549/physiolres.933938</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Participation of opioid receptors in the cytoprotective effect of chronic normobaric hypoxia

Popis výsledku v původním jazyce

We studied the role of the delta, mu, and kappa opioid receptor (OR) subtypes in the cardioprotective effect of chronic continuous normobaric hypoxia (CNH) in the model of acuteanoxia/ reoxygenation of isolated cardiomyocytes. Adaptation of rats to CNH was performed by their exposure to atmosphere containing 12 % of O-2 for 21 days. Anoxia/reoxygenation of cardiomyocytes isolated from normoxic control rats caused the death of 51 % of cells and lactate dehydrogenase (LDH) release. Adaptation of rats to CNH resulted in the anoxia/reoxygenation-induced cardiomyocyte death of only 38 %, and reduced the LDH release. Pre-incubation of the cells with either the non-selective OR blocker naloxone (300 nM/l), the delta OR antagonist TIPP(Psi) (30 nM/l), the selective delta(2) OR antagonist naltriben (1 nM/l) or the mu OR antagonist CTAP (100 nM/l) for 25 minutes before anoxia abolished the reduction of cell death and LDH release afforded by CNH. The antagonist of delta(1) OR BNTX (1 nM/l) or the kappa OR antagonist nor-binaltorphimine (3 nM/l) did not influence the cytoprotective effects of CNH. Taken together, the cytoprotective effect of CNH is associated with the activation of the delta(2 )and mu OR localized on cardiomyocytes.

Název v anglickém jazyce

Participation of opioid receptors in the cytoprotective effect of chronic normobaric hypoxia

Popis výsledku anglicky

We studied the role of the delta, mu, and kappa opioid receptor (OR) subtypes in the cardioprotective effect of chronic continuous normobaric hypoxia (CNH) in the model of acuteanoxia/ reoxygenation of isolated cardiomyocytes. Adaptation of rats to CNH was performed by their exposure to atmosphere containing 12 % of O-2 for 21 days. Anoxia/reoxygenation of cardiomyocytes isolated from normoxic control rats caused the death of 51 % of cells and lactate dehydrogenase (LDH) release. Adaptation of rats to CNH resulted in the anoxia/reoxygenation-induced cardiomyocyte death of only 38 %, and reduced the LDH release. Pre-incubation of the cells with either the non-selective OR blocker naloxone (300 nM/l), the delta OR antagonist TIPP(Psi) (30 nM/l), the selective delta(2) OR antagonist naltriben (1 nM/l) or the mu OR antagonist CTAP (100 nM/l) for 25 minutes before anoxia abolished the reduction of cell death and LDH release afforded by CNH. The antagonist of delta(1) OR BNTX (1 nM/l) or the kappa OR antagonist nor-binaltorphimine (3 nM/l) did not influence the cytoprotective effects of CNH. Taken together, the cytoprotective effect of CNH is associated with the activation of the delta(2 )and mu OR localized on cardiomyocytes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30105 - Physiology (including cytology)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Research

ISSN

0862-8408

e-ISSN

—

Svazek periodika

68

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

245-253

Kód UT WoS článku

000465948800010

EID výsledku v databázi Scopus

2-s2.0-85065473920