Enzymatic Sensor of Putrescine with Optical Oxygen Transducer - Mathematical Model of Responses of Sensitive Layer.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F15%3A00438314" target="_blank" >RIV/67985858:_____/15:00438314 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/15:33156957 RIV/60461373:22340/15:43900196

Výsledek na webu

<a href="http://dx.doi.org/10.1515/chempap-2015-0041" target="_blank" >http://dx.doi.org/10.1515/chempap-2015-0041</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/chempap-2015-0041" target="_blank" >10.1515/chempap-2015-0041</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enzymatic Sensor of Putrescine with Optical Oxygen Transducer - Mathematical Model of Responses of Sensitive Layer.

Popis výsledku v původním jazyce

A biosensor for putrescine containing a sensing layer with an optical oxygen probe based on ruthenium complex and the enzyme diamine oxidase from pea is described. The diamine oxidase was pre-immobilised on broken micro-beads modified with a ferrofluid.The pre-immobilised enzyme and ruthenium complex were both incorporated into the UV-cured inorganic?organic hybrid polymer ORMOCER and deposited on a lens to form a sensitive layer of 210 ?m in thickness. The sensitivity to the putrescine concentration determined under air saturation was between 3.50 ?s L mmol-1 and 4.50 ?s L mmol-1 in a hundred experiments conducted intermittently over a one year period. With the oxygen concentration increasing from 10 % to 100 % of DO (dissolved oxygen), the biosensorsensitivity decreased from 6.87 ?s L mmol-1 to 0.70 ?s L mmol-1 and its dynamic range increased from 0.10 mmol L-1 to 1.75 mmol L-1. To estimate the behaviour of the putrescine sensor in parametric space, a mathematical model of the reac

Název v anglickém jazyce

Enzymatic Sensor of Putrescine with Optical Oxygen Transducer - Mathematical Model of Responses of Sensitive Layer.

Popis výsledku anglicky

A biosensor for putrescine containing a sensing layer with an optical oxygen probe based on ruthenium complex and the enzyme diamine oxidase from pea is described. The diamine oxidase was pre-immobilised on broken micro-beads modified with a ferrofluid.The pre-immobilised enzyme and ruthenium complex were both incorporated into the UV-cured inorganic?organic hybrid polymer ORMOCER and deposited on a lens to form a sensitive layer of 210 ?m in thickness. The sensitivity to the putrescine concentration determined under air saturation was between 3.50 ?s L mmol-1 and 4.50 ?s L mmol-1 in a hundred experiments conducted intermittently over a one year period. With the oxygen concentration increasing from 10 % to 100 % of DO (dissolved oxygen), the biosensorsensitivity decreased from 6.87 ?s L mmol-1 to 0.70 ?s L mmol-1 and its dynamic range increased from 0.10 mmol L-1 to 1.75 mmol L-1. To estimate the behaviour of the putrescine sensor in parametric space, a mathematical model of the reac

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemical Papers

ISSN

0366-6352

e-ISSN

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Svazek periodika

69

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

9

Strana od-do

158-166

Kód UT WoS článku

000350130100014

EID výsledku v databázi Scopus

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