Analytical Characteristics of a Glucose Sensor with an Optical oxygen Transducer for the Monitoring of Biotechnological Processes.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F19%3A00512136" target="_blank" >RIV/67985858:_____/19:00512136 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22340/19:43918589

Výsledek na webu

<a href="http://hdl.handle.net/11104/0302346" target="_blank" >http://hdl.handle.net/11104/0302346</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1361-6501/aaebee" target="_blank" >10.1088/1361-6501/aaebee</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Analytical Characteristics of a Glucose Sensor with an Optical oxygen Transducer for the Monitoring of Biotechnological Processes.

Popis výsledku v původním jazyce

In this work, the analytical characteristics of the enzyme sensors of glucose with an optical oxygen transducer embedded in organic-inorganic polymer ORMOCER ® (GOR) were studied. The robust sensor was proposed for the online monitoring of biotechnological processes. The scope of the thickness, ratio of the components of the optically sensitive layers and glucose oxidase concentration in the sensitive layers with particular structure was limited by the mechanical stability of the layers and the sensitivity of the electro-optical instrumentation. These changes resulted in GOR with the sensitivity from 0.001-0.452 µs • l mmol -1 and linear dynamic range (LDR) from 0-75.0 mM. The fastest response of the prepared biosensors was 1.6 min. A further extension of the range of analytical characteristics was realized by changing the concentration of dissolved oxygen (DO) in the measured solutions. The LDR was increased more than ten times by increasing the DO from 5% to 100%. In order to estimate the analytical characteristics of the GOR sensor in the parametrical space, a mathematical model of the reaction-transport phenomena inside the optically sensitive layers was developed. This model, consisting of two reaction-diffusion equations for the glucose and oxygen substrates, was solved numerically. The dependence of the dynamics of the glucose and oxygen concentration in the sensitive layers on two dimensionless parameters was studied. One of them represents the ratio of the characteristic rates of the diffusion transport and enzyme reaction. This parameter couples the effects of the thickness of the layers and enzyme activity. The other parameter depends on the diffusivities of glucose and oxygen in the sensitive layers. The theoretical findings corresponded to the experimental data. Thus, the results of the mathematical modelling may serve as guidelines in the design of GOR and other enzyme sensors with optical oxygen transducers.

Název v anglickém jazyce

Analytical Characteristics of a Glucose Sensor with an Optical oxygen Transducer for the Monitoring of Biotechnological Processes.

Popis výsledku anglicky

In this work, the analytical characteristics of the enzyme sensors of glucose with an optical oxygen transducer embedded in organic-inorganic polymer ORMOCER ® (GOR) were studied. The robust sensor was proposed for the online monitoring of biotechnological processes. The scope of the thickness, ratio of the components of the optically sensitive layers and glucose oxidase concentration in the sensitive layers with particular structure was limited by the mechanical stability of the layers and the sensitivity of the electro-optical instrumentation. These changes resulted in GOR with the sensitivity from 0.001-0.452 µs • l mmol -1 and linear dynamic range (LDR) from 0-75.0 mM. The fastest response of the prepared biosensors was 1.6 min. A further extension of the range of analytical characteristics was realized by changing the concentration of dissolved oxygen (DO) in the measured solutions. The LDR was increased more than ten times by increasing the DO from 5% to 100%. In order to estimate the analytical characteristics of the GOR sensor in the parametrical space, a mathematical model of the reaction-transport phenomena inside the optically sensitive layers was developed. This model, consisting of two reaction-diffusion equations for the glucose and oxygen substrates, was solved numerically. The dependence of the dynamics of the glucose and oxygen concentration in the sensitive layers on two dimensionless parameters was studied. One of them represents the ratio of the characteristic rates of the diffusion transport and enzyme reaction. This parameter couples the effects of the thickness of the layers and enzyme activity. The other parameter depends on the diffusivities of glucose and oxygen in the sensitive layers. The theoretical findings corresponded to the experimental data. Thus, the results of the mathematical modelling may serve as guidelines in the design of GOR and other enzyme sensors with optical oxygen transducers.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Measurement Science and Technology

ISSN

0957-0233

e-ISSN

—

Svazek periodika

30

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

015103

Kód UT WoS článku

000452722600001

EID výsledku v databázi Scopus

2-s2.0-85063962759