ESI-TOF Mass Spectrometry of Cationic Carbosilane Dendrimers: a Potent Tool for Characterization of Structural Defects.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F18%3A00494758" target="_blank" >RIV/67985858:_____/18:00494758 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jms.4269" target="_blank" >http://dx.doi.org/10.1002/jms.4269</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jms.4269" target="_blank" >10.1002/jms.4269</a>

Jazyk výsledku

angličtina

Název v původním jazyce

ESI-TOF Mass Spectrometry of Cationic Carbosilane Dendrimers: a Potent Tool for Characterization of Structural Defects.

Popis výsledku v původním jazyce

Macromolecular polyelectrolytes are gaining considerable attention for the application in medicine which implies their detailed characterization. We have successfully applied electrospray ionization mass spectrometry (ESI MS) to the analysis of defects in the structure of three generations of polycationic carbosilane dendrimers bearing series of quarternary phosphonium groups at their periphery. Besides expected defects caused by incomplete conversion of particular reaction steps during the synthesis of dendritic scaffold and subsequent peripheral functionalization, also several products of side reactions were observed together with defects created in the course of measurement (particularly ion exchange products). Defective molecules can be to some extent separated on GPC column which proves that they are not products of in source fragmentation processes. Within the reaction sequence used for the synthesis of dendrimers under study, hydrosilylation was the source of most defects. The effectivity of quarternization depends on the type of phosphine. Results confirm high sensitivity of ESI MS towards defects, stability of the carbosilane skeleton towards fragmentation under the conditions of ESI ionization and capability to detect both lower‐ and higher molecular weight impurities arising from the synthetic sequence in the same m/z range as the target dendrimer, thus providing valuable view of the polydispersity.

Název v anglickém jazyce

ESI-TOF Mass Spectrometry of Cationic Carbosilane Dendrimers: a Potent Tool for Characterization of Structural Defects.

Popis výsledku anglicky

Macromolecular polyelectrolytes are gaining considerable attention for the application in medicine which implies their detailed characterization. We have successfully applied electrospray ionization mass spectrometry (ESI MS) to the analysis of defects in the structure of three generations of polycationic carbosilane dendrimers bearing series of quarternary phosphonium groups at their periphery. Besides expected defects caused by incomplete conversion of particular reaction steps during the synthesis of dendritic scaffold and subsequent peripheral functionalization, also several products of side reactions were observed together with defects created in the course of measurement (particularly ion exchange products). Defective molecules can be to some extent separated on GPC column which proves that they are not products of in source fragmentation processes. Within the reaction sequence used for the synthesis of dendrimers under study, hydrosilylation was the source of most defects. The effectivity of quarternization depends on the type of phosphine. Results confirm high sensitivity of ESI MS towards defects, stability of the carbosilane skeleton towards fragmentation under the conditions of ESI ionization and capability to detect both lower‐ and higher molecular weight impurities arising from the synthetic sequence in the same m/z range as the target dendrimer, thus providing valuable view of the polydispersity.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA15-05903S" target="_blank" >GA15-05903S: Nové karbosilanové dendrimery pro biomedicínské aplikace - interakce s biomolekulami a biomembránami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Mass Spectrometry

ISSN

1076-5174

e-ISSN

—

Svazek periodika

53

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

986-996

Kód UT WoS článku

000446836600010

EID výsledku v databázi Scopus

2-s2.0-85052430394