Enhancement of affinity-based biosensors: effect of sensing chamber geometry on sensitivity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F13%3A00396513" target="_blank" >RIV/67985882:_____/13:00396513 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1039/c2lc41184a" target="_blank" >http://dx.doi.org/10.1039/c2lc41184a</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c2lc41184a" target="_blank" >10.1039/c2lc41184a</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enhancement of affinity-based biosensors: effect of sensing chamber geometry on sensitivity

Popis výsledku v původním jazyce

Affinity-based biosensing systems have become an important analytical tool for the detection and study of numerous biomolecules. The merging of these sensing technologies with microfluidic flow cells allows for faster detection times, increased sensitivities, and lower required sample volumes. In order to obtain a higher degree of performance from the sensor, it is important to know the effects of the flow cell geometry on the sensor sensitivity. In these sensors, the sensor sensitivity is related to the overall diffusive flux of analyte to the sensing surface; therefore increases in the analyte flux will be manifested as an increase in sensitivity, resulting in a lower limit of detection (LOD). Here we present a study pertaining to the effects of theflow cell height H on the analyte flux J, where for a common biosensor design we predict that the analyte flux will scale as J approximate to H-2/3. We verify this scaling behavior via both numerical simulations as well as an experimental

Název v anglickém jazyce

Enhancement of affinity-based biosensors: effect of sensing chamber geometry on sensitivity

Popis výsledku anglicky

Affinity-based biosensing systems have become an important analytical tool for the detection and study of numerous biomolecules. The merging of these sensing technologies with microfluidic flow cells allows for faster detection times, increased sensitivities, and lower required sample volumes. In order to obtain a higher degree of performance from the sensor, it is important to know the effects of the flow cell geometry on the sensor sensitivity. In these sensors, the sensor sensitivity is related to the overall diffusive flux of analyte to the sensing surface; therefore increases in the analyte flux will be manifested as an increase in sensitivity, resulting in a lower limit of detection (LOD). Here we present a study pertaining to the effects of theflow cell height H on the analyte flux J, where for a common biosensor design we predict that the analyte flux will scale as J approximate to H-2/3. We verify this scaling behavior via both numerical simulations as well as an experimental

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BH - Optika, masery a lasery

OECD FORD obor

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Projekt

<a href="/cs/project/GBP205%2F12%2FG118" target="_blank" >GBP205/12/G118: Nanobiofotonika pro medicínu budoucnosti</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Lab on a Chip

ISSN

1473-0197

e-ISSN

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Svazek periodika

13

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

1413-1421

Kód UT WoS článku

000315688500025

EID výsledku v databázi Scopus

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