A Route to Superior Performance of a Nanoplasmonic Biosensor: Consideration of Both Photonic and Mass Transport Aspects

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F18%3A00490475" target="_blank" >RIV/67985882:_____/18:00490475 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1021/acsphotonics.7b01319" target="_blank" >http://dx.doi.org/10.1021/acsphotonics.7b01319</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsphotonics.7b01319" target="_blank" >10.1021/acsphotonics.7b01319</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A Route to Superior Performance of a Nanoplasmonic Biosensor: Consideration of Both Photonic and Mass Transport Aspects

Popis výsledku v původním jazyce

Optical biosensors based on plasmonic nano structures present a promising alternative to conventional biosensing methods and provide unmatched possibilities for miniaturization and high-throughput analysis. Previous works on the topic, however, have been overwhelmingly directed toward elucidating the optical performance of such sensors, with little emphasis on the topic of mass transport. To date, there exists no examination, experimental nor theoretical, of the bioanalytical performance of such sensors (in terms of detection limits) that simultaneously addresses both optical and mass transport aspects in a quantitative manner. In this work we present a universal model that describes the smallest concentration that can be detected by a nanoplasmonic biosensor. Accounting for both optical and mass transport aspects, this model establishes a relationship between bioanalytical performance and the biosensor's design parameters. We employ the model to optimize the performance of a nanoplasmonic DNA biosensor consisting of randomly distributed gold nanorods on a glass substrate. Through both experimental and theoretical results, we show that the proper design of a nanostructured sensing substrate is one that maximizes mass transport efficiency while preserving the quality of the optical readout. All results are compared with those obtained using a conventional SPR biosensor. We show that an optimized nanoplasmonic substrate allows for the detection of DNA at concentrations of an order of magnitude lower with respect to an SPR biosensor

Název v anglickém jazyce

A Route to Superior Performance of a Nanoplasmonic Biosensor: Consideration of Both Photonic and Mass Transport Aspects

Popis výsledku anglicky

Optical biosensors based on plasmonic nano structures present a promising alternative to conventional biosensing methods and provide unmatched possibilities for miniaturization and high-throughput analysis. Previous works on the topic, however, have been overwhelmingly directed toward elucidating the optical performance of such sensors, with little emphasis on the topic of mass transport. To date, there exists no examination, experimental nor theoretical, of the bioanalytical performance of such sensors (in terms of detection limits) that simultaneously addresses both optical and mass transport aspects in a quantitative manner. In this work we present a universal model that describes the smallest concentration that can be detected by a nanoplasmonic biosensor. Accounting for both optical and mass transport aspects, this model establishes a relationship between bioanalytical performance and the biosensor's design parameters. We employ the model to optimize the performance of a nanoplasmonic DNA biosensor consisting of randomly distributed gold nanorods on a glass substrate. Through both experimental and theoretical results, we show that the proper design of a nanostructured sensing substrate is one that maximizes mass transport efficiency while preserving the quality of the optical readout. All results are compared with those obtained using a conventional SPR biosensor. We show that an optimized nanoplasmonic substrate allows for the detection of DNA at concentrations of an order of magnitude lower with respect to an SPR biosensor

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/GA15-06785S" target="_blank" >GA15-06785S: Regulační úseky nukleových kyselin - polymorfismus, dynamika a interakce</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS Photonics

ISSN

2330-4022

e-ISSN

—

Svazek periodika

5

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

1019-1025

Kód UT WoS článku

000428356400047

EID výsledku v databázi Scopus

2-s2.0-85044317759