Multiscale modeling and analysis for high-fidelity interferometric scattering microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F21%3A00543290" target="_blank" >RIV/67985882:_____/21:00543290 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1088/1361-6463/abf70d" target="_blank" >https://doi.org/10.1088/1361-6463/abf70d</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1361-6463/abf70d" target="_blank" >10.1088/1361-6463/abf70d</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multiscale modeling and analysis for high-fidelity interferometric scattering microscopy

Popis výsledku v původním jazyce

Interferometric scattering microscopy (iSCAT), as an ultrasensitive fluorescence-free imaging modality, has recently gained enormous attention and has been rapidly developing from demonstration of principle to quantitative sensing and bioanalytics. Here we report on a theoretical framework of multiscale modeling and analysis for iSCAT with samples of arbitrary shapes under any types of illumination and detection schemes. We theoretically predict and experimentally confirm different evolution behaviors of the interference contrast as a function of the axial defocusing for dielectric and metallic nanoparticles. We provide a transparent understanding of the origin of the interference phenomenon in terms of plane wave components and explain how the interference contrast changes with the size and material of the nanoprobe and the numerical aperture of the microscope objective. Moreover, we investigate a sample system mimicking a gold nanoparticle in a simplified cell environment and show the position-dependent and asymmetric point spread function of the nanoparticle

Název v anglickém jazyce

Multiscale modeling and analysis for high-fidelity interferometric scattering microscopy

Popis výsledku anglicky

Interferometric scattering microscopy (iSCAT), as an ultrasensitive fluorescence-free imaging modality, has recently gained enormous attention and has been rapidly developing from demonstration of principle to quantitative sensing and bioanalytics. Here we report on a theoretical framework of multiscale modeling and analysis for iSCAT with samples of arbitrary shapes under any types of illumination and detection schemes. We theoretically predict and experimentally confirm different evolution behaviors of the interference contrast as a function of the axial defocusing for dielectric and metallic nanoparticles. We provide a transparent understanding of the origin of the interference phenomenon in terms of plane wave components and explain how the interference contrast changes with the size and material of the nanoprobe and the numerical aperture of the microscope objective. Moreover, we investigate a sample system mimicking a gold nanoparticle in a simplified cell environment and show the position-dependent and asymmetric point spread function of the nanoparticle

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Physics D-Applied Physics

ISSN

0022-3727

e-ISSN

1361-6463

Svazek periodika

54

Číslo periodika v rámci svazku

27

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

274002

Kód UT WoS článku

000644688700001

EID výsledku v databázi Scopus

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