EFFECT OF DIETARY SUPPLEMENTATION WITH DRIED TUBER OF JERUSALEM ARTICHOKE ON SKATOLE LEVEL IN BACKFAT AND CYP2E1 mRNA EXPRESSION IN LIVER OF BOARS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F21%3A00550739" target="_blank" >RIV/67985904:_____/21:00550739 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/21:88314

Výsledek na webu

<a href="https://www.sciendo.com/article/10.2478/aoas-2020-0119" target="_blank" >https://www.sciendo.com/article/10.2478/aoas-2020-0119</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/aoas-2020-0119" target="_blank" >10.2478/aoas-2020-0119</a>

Jazyk výsledku

angličtina

Název v původním jazyce

EFFECT OF DIETARY SUPPLEMENTATION WITH DRIED TUBER OF JERUSALEM ARTICHOKE ON SKATOLE LEVEL IN BACKFAT AND CYP2E1 mRNA EXPRESSION IN LIVER OF BOARS

Popis výsledku v původním jazyce

The objective of this study was to investigate the effect of diets containing different levels of dried tuber of Jerusalem artichoke, Helianthus tuberosus, on skatole levels in backfat and on the CYP2E1 mRNA expression in the liver of commercial crossbred pigs. A total of 23 uncastrated male pigs from 10 litters of a commercial crossbred population of Large White x (Landrace x Large White), were used in this study. Boars were randomly divided into four different dietary treatment groups - a control group (K1, 5 boars, without supplementation of Jerusalem artichoke) and three experimental groups (6 boars each) that were fed with the diet containing different levels of dried Jerusalem artichoke (K2 - 4.1%, K3 - 8.2%, K4 - 12.2%) for 14 days before slaughter. Significant effects of diet on skatole levels were observed between the control group and the experimental groups (P = 0.0078). The lowest level of skatole was in the K3 group with 8.2% of Jerusalem artichoke. As for CYP2E1, a negative correlation was observed between the levels of skatole and CYP2E1 mRNA expression. Significant effect (P = 0.0055) was found in all experimental groups compared to the K1 group, and most pronounced in the K2 and K3 groups. The supplementation with Jerusalem artichoke resulted in lower level of skatole and higher CYP2E1 mRNA expression. The results suggest that affecting the expression of CYP2E1 by feed supplements could be an option to effectively reduce the levels of skatole in adipose tissue of entire male pigs.

Název v anglickém jazyce

EFFECT OF DIETARY SUPPLEMENTATION WITH DRIED TUBER OF JERUSALEM ARTICHOKE ON SKATOLE LEVEL IN BACKFAT AND CYP2E1 mRNA EXPRESSION IN LIVER OF BOARS

Popis výsledku anglicky

The objective of this study was to investigate the effect of diets containing different levels of dried tuber of Jerusalem artichoke, Helianthus tuberosus, on skatole levels in backfat and on the CYP2E1 mRNA expression in the liver of commercial crossbred pigs. A total of 23 uncastrated male pigs from 10 litters of a commercial crossbred population of Large White x (Landrace x Large White), were used in this study. Boars were randomly divided into four different dietary treatment groups - a control group (K1, 5 boars, without supplementation of Jerusalem artichoke) and three experimental groups (6 boars each) that were fed with the diet containing different levels of dried Jerusalem artichoke (K2 - 4.1%, K3 - 8.2%, K4 - 12.2%) for 14 days before slaughter. Significant effects of diet on skatole levels were observed between the control group and the experimental groups (P = 0.0078). The lowest level of skatole was in the K3 group with 8.2% of Jerusalem artichoke. As for CYP2E1, a negative correlation was observed between the levels of skatole and CYP2E1 mRNA expression. Significant effect (P = 0.0055) was found in all experimental groups compared to the K1 group, and most pronounced in the K2 and K3 groups. The supplementation with Jerusalem artichoke resulted in lower level of skatole and higher CYP2E1 mRNA expression. The results suggest that affecting the expression of CYP2E1 by feed supplements could be an option to effectively reduce the levels of skatole in adipose tissue of entire male pigs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Annals of Animal Science

ISSN

1642-3402

e-ISSN

2300-8733

Svazek periodika

21

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

15

Strana od-do

1475-1489

Kód UT WoS článku

000729942700017

EID výsledku v databázi Scopus

2-s2.0-85105608949