Short-term holding of female broodstock at improper spawning temperature can trigger the incidence of autotriploidy in sterlet ( Acipenser ruthenus )

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F24%3A00587910" target="_blank" >RIV/67985904:_____/24:00587910 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12520/24:43908157

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0044848624006963?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0044848624006963?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2024.741235" target="_blank" >10.1016/j.aquaculture.2024.741235</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Short-term holding of female broodstock at improper spawning temperature can trigger the incidence of autotriploidy in sterlet ( Acipenser ruthenus )

Popis výsledku v původním jazyce

Artificial propagation is an essential source of sturgeon progeny for both commercial and conservation purposes. However, in culture settings sturgeons often spontaneously produce offspring with altered ploidy - mainly autotriploids having in somatic cells 1.5-fold more DNA than fish of normal ploidy. The presence of autotriploids rather than individuals of normal ploidy may decrease farm productivity or wild population recovery. We tested whether or not the temperature for holding female broodstock contributes to the incidence of spontaneous autotriploidy in sterlet (Acipenser ruthenus). We compared the occurrence of autotriploidy in the progeny of females held at various temperatures ranging from the optimal range for sterlet reproduction (12, 14 and 16 degrees C), in the progeny of females held at optimal and two above-optimal temperatures (15, 18 and 20 degrees C) and in the progeny of females held at optimal and below-optimal temperature (15 and 10 degrees C). The respective temperatures were reached before hormonal stimulation and kept until egg collection. Eggs were stripped within 2 h postovulation and fertilized within 30 min, ploidy was analysed in prelarvae sampled 2-3 d post-hatching. Only low proportions of autotriploids (0-1.1%) were detected in the progeny of females held at 10, 12, 14 and 16 degrees C, which was the case also for females held at 15 degrees C. However, a considerable number of females from aboveoptimal temperatures (two out of three females at 20 degrees C and at least two out of six females at 18 degrees C) produced significantly higher proportions of autotriploids, with the highest proportion observed in a female held at 20 degrees C: 53.3%. We used water at various temperatures for the fertilization of eggs and incubation of embryos: when each of the female-holding temperatures 12, 14 and 16 degrees C was combined with 12, 14 and 16 degrees C for fertilization and incubation, the holding temperatures 15, 18 and 20 degrees C were combined with 15, 18 and 20 degrees C, and holding temperatures 10 and 15 degrees C were combined with 10 and 15 degrees C, the proportions of spontaneous autotriploids remained statistically unchanged. We performed microsatellite analysis on autotriploid progeny of some females held at above-optimal temperatures and confirmed maternal origin of the third chromosome set. Apart from autotriploids, other individuals of abnormal ploidy were detected (tetraploids, pentaploids, haplodiploid, haplo-triploid, diplo-triploid and diplo-tetraploid mosaics), whose occurrence seemed to be low and randomly distributed. We conclude that even the short-term holding (2-day) of sterlet female broodstock at temperatures of 18 degrees C or higher can often result in a remarkable occurrence of spontaneously autotriploid progeny, while the holding of females at 10-16 degrees C and/or using of water at 10-20 degrees C for fertilization and incubation do not seem to affect the incidence of autotriploidy.

Název v anglickém jazyce

Short-term holding of female broodstock at improper spawning temperature can trigger the incidence of autotriploidy in sterlet ( Acipenser ruthenus )

Popis výsledku anglicky

Artificial propagation is an essential source of sturgeon progeny for both commercial and conservation purposes. However, in culture settings sturgeons often spontaneously produce offspring with altered ploidy - mainly autotriploids having in somatic cells 1.5-fold more DNA than fish of normal ploidy. The presence of autotriploids rather than individuals of normal ploidy may decrease farm productivity or wild population recovery. We tested whether or not the temperature for holding female broodstock contributes to the incidence of spontaneous autotriploidy in sterlet (Acipenser ruthenus). We compared the occurrence of autotriploidy in the progeny of females held at various temperatures ranging from the optimal range for sterlet reproduction (12, 14 and 16 degrees C), in the progeny of females held at optimal and two above-optimal temperatures (15, 18 and 20 degrees C) and in the progeny of females held at optimal and below-optimal temperature (15 and 10 degrees C). The respective temperatures were reached before hormonal stimulation and kept until egg collection. Eggs were stripped within 2 h postovulation and fertilized within 30 min, ploidy was analysed in prelarvae sampled 2-3 d post-hatching. Only low proportions of autotriploids (0-1.1%) were detected in the progeny of females held at 10, 12, 14 and 16 degrees C, which was the case also for females held at 15 degrees C. However, a considerable number of females from aboveoptimal temperatures (two out of three females at 20 degrees C and at least two out of six females at 18 degrees C) produced significantly higher proportions of autotriploids, with the highest proportion observed in a female held at 20 degrees C: 53.3%. We used water at various temperatures for the fertilization of eggs and incubation of embryos: when each of the female-holding temperatures 12, 14 and 16 degrees C was combined with 12, 14 and 16 degrees C for fertilization and incubation, the holding temperatures 15, 18 and 20 degrees C were combined with 15, 18 and 20 degrees C, and holding temperatures 10 and 15 degrees C were combined with 10 and 15 degrees C, the proportions of spontaneous autotriploids remained statistically unchanged. We performed microsatellite analysis on autotriploid progeny of some females held at above-optimal temperatures and confirmed maternal origin of the third chromosome set. Apart from autotriploids, other individuals of abnormal ploidy were detected (tetraploids, pentaploids, haplodiploid, haplo-triploid, diplo-triploid and diplo-tetraploid mosaics), whose occurrence seemed to be low and randomly distributed. We conclude that even the short-term holding (2-day) of sterlet female broodstock at temperatures of 18 degrees C or higher can often result in a remarkable occurrence of spontaneously autotriploid progeny, while the holding of females at 10-16 degrees C and/or using of water at 10-20 degrees C for fertilization and incubation do not seem to affect the incidence of autotriploidy.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10613 - Zoology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

1873-5622

Svazek periodika

592

Číslo periodika v rámci svazku

NOV 15

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

741235

Kód UT WoS článku

001259606700001

EID výsledku v databázi Scopus

2-s2.0-85196645720