Electrochemical Reduction and Oxidation of Six Natural 2 '-Deoxynucleosides at a Pyrolytic Graphite Electrode in the Presence or Absence of Ambient Oxygen

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00518363" target="_blank" >RIV/68081707:_____/19:00518363 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/19:00112928

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.201900417" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.201900417</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elan.201900417" target="_blank" >10.1002/elan.201900417</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical Reduction and Oxidation of Six Natural 2 '-Deoxynucleosides at a Pyrolytic Graphite Electrode in the Presence or Absence of Ambient Oxygen

Popis výsledku v původním jazyce

Recently we have demonstrated that pyrolytic graphite electrodes (PGE) offer a broad analytically useful potential window, ranging from about2.0 V to +1.6 V vs. Ag|AgCl|3 M KCl, which enables to use the PGE not only for anodic measurements, but also for direct reduction of nucleobases in DNA oligonucleotides. In this follow-up study, we have focused on the electrochemical behavior of four 2 '-deoxynucleosides derived from adenine, guanine, cytosine, thymine, uracil, and 5-methyl cytosine on the PGE. On one hand we have obtained analogous primary redox responses as previously with the oligonucleotides. On the other hand, significant differences were observed, particularly when considering secondary responses involving products of the primary conversions, suggesting involvement of different mechanisms. Further we have found that presence of the ambient oxygen in the electrolyte does not dramatically affect the redox signals of the nucleosides. This finding is in contrast with DNA responses measured at the mercury-based electrodes, where deaeration prior to the measurements was necessary. We demonstrate that all studied nucleosides can be analyzed using a simple ex situ (medium exchange) procedure.

Název v anglickém jazyce

Electrochemical Reduction and Oxidation of Six Natural 2 '-Deoxynucleosides at a Pyrolytic Graphite Electrode in the Presence or Absence of Ambient Oxygen

Popis výsledku anglicky

Recently we have demonstrated that pyrolytic graphite electrodes (PGE) offer a broad analytically useful potential window, ranging from about2.0 V to +1.6 V vs. Ag|AgCl|3 M KCl, which enables to use the PGE not only for anodic measurements, but also for direct reduction of nucleobases in DNA oligonucleotides. In this follow-up study, we have focused on the electrochemical behavior of four 2 '-deoxynucleosides derived from adenine, guanine, cytosine, thymine, uracil, and 5-methyl cytosine on the PGE. On one hand we have obtained analogous primary redox responses as previously with the oligonucleotides. On the other hand, significant differences were observed, particularly when considering secondary responses involving products of the primary conversions, suggesting involvement of different mechanisms. Further we have found that presence of the ambient oxygen in the electrolyte does not dramatically affect the redox signals of the nucleosides. This finding is in contrast with DNA responses measured at the mercury-based electrodes, where deaeration prior to the measurements was necessary. We demonstrate that all studied nucleosides can be analyzed using a simple ex situ (medium exchange) procedure.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

—

Svazek periodika

31

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

2057-2066

Kód UT WoS článku

000481001100001

EID výsledku v databázi Scopus

—