Slug-expressing mouse prostate epithelial cells have increased stem cell potential

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F20%3A00539347" target="_blank" >RIV/68081707:_____/20:00539347 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00159816:_____/20:00073528 RIV/00216224:14310/20:00117616 RIV/00098892:_____/20:N0000087 RIV/61989592:15110/20:73602697

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1873506120301458?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1873506120301458?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.scr.2020.101844" target="_blank" >10.1016/j.scr.2020.101844</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Slug-expressing mouse prostate epithelial cells have increased stem cell potential

Popis výsledku v původním jazyce

Deciphering the properties of adult stem cells is crucial for understanding of their role in healthy tissue and in cancer progression as well. Both stem cells and cancer stem cells have shown association with epithelial-to-mesenchymal transition (EMT) in various tissue types. Aiming to investigate the epithelial and mesenchymal phenotypic traits in adult mouse prostate, we sorted subpopulations of basal prostate stem cells (mPSCs) and assessed the expression levels of EMT regulators and markers with custom-designed gene expression array. The population of mPSCs defined by a Lin(-)/Sca-1(+) CD49f(hi)/Trop-2(+) (LSC Trop-(2+)) surface phenotype was enriched in mesenchymal markers, especially EMT master regulator Slug, encoded by the Snail gene. To further dissect the role of Slug in mPSCs, we used transgenic Snai2(tm1.1wbg) reporter mouse strain. Using this model, we confirmed the presence of mesenchymal traits and increase of organoid forming capacity in Slug(+) population of mPSCs. The Slug(+)derived organoids comprised all prostate epithelial cell types basal, luminal, and neuroendocrine. Collectively, these data uncover the important role of Slug expression in the physiology of mouse prostate stem cells.

Název v anglickém jazyce

Slug-expressing mouse prostate epithelial cells have increased stem cell potential

Popis výsledku anglicky

Deciphering the properties of adult stem cells is crucial for understanding of their role in healthy tissue and in cancer progression as well. Both stem cells and cancer stem cells have shown association with epithelial-to-mesenchymal transition (EMT) in various tissue types. Aiming to investigate the epithelial and mesenchymal phenotypic traits in adult mouse prostate, we sorted subpopulations of basal prostate stem cells (mPSCs) and assessed the expression levels of EMT regulators and markers with custom-designed gene expression array. The population of mPSCs defined by a Lin(-)/Sca-1(+) CD49f(hi)/Trop-2(+) (LSC Trop-(2+)) surface phenotype was enriched in mesenchymal markers, especially EMT master regulator Slug, encoded by the Snail gene. To further dissect the role of Slug in mPSCs, we used transgenic Snai2(tm1.1wbg) reporter mouse strain. Using this model, we confirmed the presence of mesenchymal traits and increase of organoid forming capacity in Slug(+) population of mPSCs. The Slug(+)derived organoids comprised all prostate epithelial cell types basal, luminal, and neuroendocrine. Collectively, these data uncover the important role of Slug expression in the physiology of mouse prostate stem cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Stem Cell Research

ISSN

1873-5061

e-ISSN

—

Svazek periodika

46

Číslo periodika v rámci svazku

jul 2020

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

101844

Kód UT WoS článku

000566516300010

EID výsledku v databázi Scopus

2-s2.0-85087084813