SARS-CoV-2 hot-spot mutations are significantly enriched within inverted repeats and CpG island loci

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F21%3A00555382" target="_blank" >RIV/68081707:_____/21:00555382 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61988987:17310/21:A22029UR RIV/00216224:14310/21:00121025

Výsledek na webu

<a href="https://academic.oup.com/bib/article/22/2/1338/6042389" target="_blank" >https://academic.oup.com/bib/article/22/2/1338/6042389</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/bib/bbaa385" target="_blank" >10.1093/bib/bbaa385</a>

Jazyk výsledku

angličtina

Název v původním jazyce

SARS-CoV-2 hot-spot mutations are significantly enriched within inverted repeats and CpG island loci

Popis výsledku v původním jazyce

SARS-CoV-2 is an intensively investigated virus from the order Nidovirales (Coronaviridae family) that causes COVID-19 disease in humans. Through enormous scientific effort, thousands of viral strains have been sequenced to date, thereby creating a strong background for deep bioinformatics studies of the SARS-CoV-2 genome. In this study, we inspected high-frequency mutations of SARS-CoV-2 and carried out systematic analyses of their overlay with inverted repeat (IR) loci and CpG islands. The main conclusion of our study is that SARS-CoV-2 hot-spot mutations are significantly enriched within both IRs and CpG island loci. This points to their role in genomic instability and may predict further mutational drive of the SARS-CoV-2 genome. Moreover, CpG islands are strongly enriched upstream from viral ORFs and thus could play important roles in transcription and the viral life cycle. We hypothesize that hypermethylation of these loci will decrease the transcription of viral ORFs and could therefore limit the progression of the disease.

Název v anglickém jazyce

SARS-CoV-2 hot-spot mutations are significantly enriched within inverted repeats and CpG island loci

Popis výsledku anglicky

SARS-CoV-2 is an intensively investigated virus from the order Nidovirales (Coronaviridae family) that causes COVID-19 disease in humans. Through enormous scientific effort, thousands of viral strains have been sequenced to date, thereby creating a strong background for deep bioinformatics studies of the SARS-CoV-2 genome. In this study, we inspected high-frequency mutations of SARS-CoV-2 and carried out systematic analyses of their overlay with inverted repeat (IR) loci and CpG islands. The main conclusion of our study is that SARS-CoV-2 hot-spot mutations are significantly enriched within both IRs and CpG island loci. This points to their role in genomic instability and may predict further mutational drive of the SARS-CoV-2 genome. Moreover, CpG islands are strongly enriched upstream from viral ORFs and thus could play important roles in transcription and the viral life cycle. We hypothesize that hypermethylation of these loci will decrease the transcription of viral ORFs and could therefore limit the progression of the disease.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Briefings in Bioinformatics

ISSN

1467-5463

e-ISSN

1477-4054

Svazek periodika

22

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

1338-1345

Kód UT WoS článku

000642298000058

EID výsledku v databázi Scopus

2-s2.0-85103474633