Decreased protein peak asymmetry and width due to static capillary coating with hydrophilic derivatives of polydimethylacrylamide.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F02%3A25020018" target="_blank" >RIV/68081715:_____/02:25020018 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Decreased protein peak asymmetry and width due to static capillary coating with hydrophilic derivatives of polydimethylacrylamide.

Popis výsledku v původním jazyce

Protein CZE peaks in the EPDMA-coated capillaries exhibited a peak asymmetry similar to that obtained in capillaries with 'covalent static' coating of polyacrylamide, suggesting a similar degree of adsorption of the protein onto the coating. Instabilityof such coating at very low ionic strength and its stripping from the capillary in the presence of SDS also indicated a hydrophilic bonding of EPDMA to the silanol surface of the capillary, while its stripping in the CZE of 'carboxylate-modified' polystyrene suggested a competition between carboxylate and EPDMA for the hydrophilic bonds to silanol. To test those propositions, a number of EPDMA-derived coating agents with increased hydrophilicity were syntesized. Of a number of the hydrophilic coating agents tested, only two exhibited for representative proteins a decreased peak asymmetry and width while the stability of the suppresion of EOF, and the stability of mobility in consecutive CZE runs was reduced relative to EPDMA. Coating ag

Název v anglickém jazyce

Decreased protein peak asymmetry and width due to static capillary coating with hydrophilic derivatives of polydimethylacrylamide.

Popis výsledku anglicky

Protein CZE peaks in the EPDMA-coated capillaries exhibited a peak asymmetry similar to that obtained in capillaries with 'covalent static' coating of polyacrylamide, suggesting a similar degree of adsorption of the protein onto the coating. Instabilityof such coating at very low ionic strength and its stripping from the capillary in the presence of SDS also indicated a hydrophilic bonding of EPDMA to the silanol surface of the capillary, while its stripping in the CZE of 'carboxylate-modified' polystyrene suggested a competition between carboxylate and EPDMA for the hydrophilic bonds to silanol. To test those propositions, a number of EPDMA-derived coating agents with increased hydrophilicity were syntesized. Of a number of the hydrophilic coating agents tested, only two exhibited for representative proteins a decreased peak asymmetry and width while the stability of the suppresion of EOF, and the stability of mobility in consecutive CZE runs was reduced relative to EPDMA. Coating ag

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

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Svazek periodika

23

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

2274-2278

Kód UT WoS článku

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EID výsledku v databázi Scopus

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