Fast blood plasma separation device for point-of-care applications

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F18%3A00486643" target="_blank" >RIV/68081715:_____/18:00486643 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/18:00111646

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.talanta.2018.02.004" target="_blank" >http://dx.doi.org/10.1016/j.talanta.2018.02.004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2018.02.004" target="_blank" >10.1016/j.talanta.2018.02.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fast blood plasma separation device for point-of-care applications

Popis výsledku v původním jazyce

In this work, a simple device for extremely fast separation of blood plasma from diluted whole blood was developed.nThe device accommodates an asymmetric polysulfone membrane/supporting membrane sandwich thatnallows collection of 10μL blood plasma into a narrow glass capillary in less than 10s. The composition of diluentnsolution was optimized in order to achieve maximum recoveries for selected metabolites of alcohol intoxication.n5% solution of [tris(hydroxymethyl)methylamino] propanesulfonic acid provided recoveries of formate, oxalatenand glycolate close to 100% and only moderate erythrocyte lysis. Both charged and uncharged compounds fromnthe whole blood samples can be analyzed in the separated blood plasma by capillary electrophoresis with contactlessnconductometric detection and spectrophotometry, respectively. The developed device might find widenapplication in on-site testing and point-of-care analysis, when only microliter volumes of whole blood are available.

Název v anglickém jazyce

Fast blood plasma separation device for point-of-care applications

Popis výsledku anglicky

In this work, a simple device for extremely fast separation of blood plasma from diluted whole blood was developed.nThe device accommodates an asymmetric polysulfone membrane/supporting membrane sandwich thatnallows collection of 10μL blood plasma into a narrow glass capillary in less than 10s. The composition of diluentnsolution was optimized in order to achieve maximum recoveries for selected metabolites of alcohol intoxication.n5% solution of [tris(hydroxymethyl)methylamino] propanesulfonic acid provided recoveries of formate, oxalatenand glycolate close to 100% and only moderate erythrocyte lysis. Both charged and uncharged compounds fromnthe whole blood samples can be analyzed in the separated blood plasma by capillary electrophoresis with contactlessnconductometric detection and spectrophotometry, respectively. The developed device might find widenapplication in on-site testing and point-of-care analysis, when only microliter volumes of whole blood are available.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

—

Svazek periodika

183

Číslo periodika v rámci svazku

JUN

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

55-60

Kód UT WoS článku

000430645800008

EID výsledku v databázi Scopus

2-s2.0-85042222134