Sub-minute analysis of lactate from a single blood drop using capillary electrophoresis with contactless conductivity detection in monitoring of athlete performance

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F21%3A00546268" target="_blank" >RIV/68081715:_____/21:00546268 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/21:00124349

Výsledek na webu

<a href="https://www.mdpi.com/1420-3049/26/19/5817" target="_blank" >https://www.mdpi.com/1420-3049/26/19/5817</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/molecules26195817" target="_blank" >10.3390/molecules26195817</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sub-minute analysis of lactate from a single blood drop using capillary electrophoresis with contactless conductivity detection in monitoring of athlete performance

Popis výsledku v původním jazyce

A simple and fast method for the analysis of lactate from a single drop of blood was developed. The finger-prick whole blood sample (10 µL) was diluted (1:20) with a 7% (w/v) solution of tris(hydroxymethyl)methylamino] propanesulfonic acid and applied to a blood plasma separation device. The device accommodates a membrane sandwich composed of an asymmetric polysulfone membrane and a supporting textile membrane that allows the collection of blood plasma into a narrow glass capillary in less than 20 s. Separated and simultaneously diluted blood plasma was directly injected into a capillary electrophoresis instrument with a contactless conductivity detector (CE-C4 D) and analyzed in less than one minute. A separation electrolyte consisted of 10 mmol/L L-histidine, 15 mmol/L DL-glutamic acid, and 30 µmol/L cetyltrimethylammonium bromide. The whole procedure starting from the finger-prick sampling until the CE-C4 D analysis was finished, took less than 5 min and was suitable for monitoring lactate increase in blood plasma during incremental cycling exercise. The observed lactate increase during the experiments measured by the developed CE-C4 D method correlated well with the results from a hand-held lactate analyzer (R = 0.9882). The advantage of the developed CE method is the speed, significant savings per analysis, and the possibility to analyze other compounds from blood plasma

Název v anglickém jazyce

Sub-minute analysis of lactate from a single blood drop using capillary electrophoresis with contactless conductivity detection in monitoring of athlete performance

Popis výsledku anglicky

A simple and fast method for the analysis of lactate from a single drop of blood was developed. The finger-prick whole blood sample (10 µL) was diluted (1:20) with a 7% (w/v) solution of tris(hydroxymethyl)methylamino] propanesulfonic acid and applied to a blood plasma separation device. The device accommodates a membrane sandwich composed of an asymmetric polysulfone membrane and a supporting textile membrane that allows the collection of blood plasma into a narrow glass capillary in less than 20 s. Separated and simultaneously diluted blood plasma was directly injected into a capillary electrophoresis instrument with a contactless conductivity detector (CE-C4 D) and analyzed in less than one minute. A separation electrolyte consisted of 10 mmol/L L-histidine, 15 mmol/L DL-glutamic acid, and 30 µmol/L cetyltrimethylammonium bromide. The whole procedure starting from the finger-prick sampling until the CE-C4 D analysis was finished, took less than 5 min and was suitable for monitoring lactate increase in blood plasma during incremental cycling exercise. The observed lactate increase during the experiments measured by the developed CE-C4 D method correlated well with the results from a hand-held lactate analyzer (R = 0.9882). The advantage of the developed CE method is the speed, significant savings per analysis, and the possibility to analyze other compounds from blood plasma

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecules

ISSN

1420-3049

e-ISSN

1420-3049

Svazek periodika

26

Číslo periodika v rámci svazku

19

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

9

Strana od-do

5817

Kód UT WoS článku

000717228700001

EID výsledku v databázi Scopus

2-s2.0-85115829560