Influence of water environment on biological specimens imaging in ESEM.

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Influence of water environment on biological specimens imaging in ESEM.

Popis výsledku v původním jazyce

Environmental scanning electron microscope (ESEM) allows the visualisation of specimens that are difficult or impossible to image in a conventional high vacuum SEM. Pressure in the specimen chamber ranges from 1 Pa over 2000 Pa, which permits observationof specimens of being free from charging artefacts and containing different volume of water. But there are some difficulties with the observation of highly wet biological specimens in their natural state because during a pre-pumping process of the specimen chamber an escape of water from the specimen occurs and thus its surface is damaged. The aim of this contribution is to find a method making possible to decrease water escape from the specimen during the initiation pumping process of the ESEM, immediatelly after the inserting of the specimen into the specimen chamber and during an image recording.

Název v anglickém jazyce

Influence of water environment on biological specimens imaging in ESEM.

Popis výsledku anglicky

Environmental scanning electron microscope (ESEM) allows the visualisation of specimens that are difficult or impossible to image in a conventional high vacuum SEM. Pressure in the specimen chamber ranges from 1 Pa over 2000 Pa, which permits observationof specimens of being free from charging artefacts and containing different volume of water. But there are some difficulties with the observation of highly wet biological specimens in their natural state because during a pre-pumping process of the specimen chamber an escape of water from the specimen occurs and thus its surface is damaged. The aim of this contribution is to find a method making possible to decrease water escape from the specimen during the initiation pumping process of the ESEM, immediatelly after the inserting of the specimen into the specimen chamber and during an image recording.

Druh

D - Stať ve sborníku

CEP obor

JA - Elektronika a optoelektronika, elektrotechnika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 6th Multinational Congress on Microscopy - European Extension.

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

497-498

Název nakladatele

Croatian Society for Electron Microscopy

Místo vydání

Zagreb

Místo konání akce

Pula [HR]

Datum konání akce

1. 6. 2003

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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