Imaging of Norway spruce early somatic embryos with the ESEM, Cryo-SEM and laser scanning microscope

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F16%3A00461452" target="_blank" >RIV/68081731:_____/16:00461452 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/16:43909356

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.micron.2016.02.011" target="_blank" >http://dx.doi.org/10.1016/j.micron.2016.02.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.micron.2016.02.011" target="_blank" >10.1016/j.micron.2016.02.011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Imaging of Norway spruce early somatic embryos with the ESEM, Cryo-SEM and laser scanning microscope

Popis výsledku v původním jazyce

This article describes the surface structure of Norway spruce early somatic embryos (ESEs) as a typical culture with asynchronous development. The microstructure of extracellular matrix covering ESEs were observed using the environmental scanning electron microscope as a primary tool and using the scanning electron microscope with cryo attachment and laser electron microscope as a complementary tool allowing our results to be proven independently. The fresh samples were observed in conditions of the air environment of the environmental scanning electron microscope (ESEM) with the pressure from 550 Pa to 690 Pa and the low temperature of the sample from -18 degrees C to -22 degrees C. The samples were studied using two different types of detector to allow studying either the thin surface structure or material composition. The scanning electron microscope with cryo attachment was used for imaging frozen extracellular matrix microstructure with higher resolution. The combination of both electron microscopy methods was suitable for observation of "native" plant samples, allowing correct evaluation of our results, free of error and artifacts.

Název v anglickém jazyce

Imaging of Norway spruce early somatic embryos with the ESEM, Cryo-SEM and laser scanning microscope

Popis výsledku anglicky

This article describes the surface structure of Norway spruce early somatic embryos (ESEs) as a typical culture with asynchronous development. The microstructure of extracellular matrix covering ESEs were observed using the environmental scanning electron microscope as a primary tool and using the scanning electron microscope with cryo attachment and laser electron microscope as a complementary tool allowing our results to be proven independently. The fresh samples were observed in conditions of the air environment of the environmental scanning electron microscope (ESEM) with the pressure from 550 Pa to 690 Pa and the low temperature of the sample from -18 degrees C to -22 degrees C. The samples were studied using two different types of detector to allow studying either the thin surface structure or material composition. The scanning electron microscope with cryo attachment was used for imaging frozen extracellular matrix microstructure with higher resolution. The combination of both electron microscopy methods was suitable for observation of "native" plant samples, allowing correct evaluation of our results, free of error and artifacts.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JA - Elektronika a optoelektronika, elektrotechnika

OECD FORD obor

—

Projekt

<a href="/cs/project/GA14-22777S" target="_blank" >GA14-22777S: Studium interakcí elektronů s plynem v podmínkách tlakového spádu nízkoenergiového environmentálního rastrovacího elektronového mikroskopu.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Micron

ISSN

0968-4328

e-ISSN

—

Svazek periodika

84

Číslo periodika v rámci svazku

May 2016

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

67-71

Kód UT WoS článku

000375167000009

EID výsledku v databázi Scopus

2-s2.0-84959269654