Individual copy number variation and extensive diversity between major MHC-DAB1 allelic lineages in the European bitterling

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F22%3A00551782" target="_blank" >RIV/68081766:_____/22:00551782 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/22:92151 RIV/00216224:14310/22:00127856

Výsledek na webu

<a href="https://link.springer.com/article/10.1007%2Fs00251-021-01251-4" target="_blank" >https://link.springer.com/article/10.1007%2Fs00251-021-01251-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00251-021-01251-4" target="_blank" >10.1007/s00251-021-01251-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Individual copy number variation and extensive diversity between major MHC-DAB1 allelic lineages in the European bitterling

Popis výsledku v původním jazyce

Polymorphism of the major histocompatibility complex (MHC), DAB1 gene was characterized for the first time in the European bitterling (Rhodeus amarus), a freshwater fish employed in studies of host-parasite coevolution and mate choice, taking advantage of newly designed primers coupled with high-throughput amplicon sequencing. Across 221 genotyped individuals, we detected 1-4 variants per fish, with 28% individuals possessing 3-4 variants. We identified 36 DAB1 variants, and they showed high sequence diversity mostly located within predicted antigen-binding sites, and both global and codon-specific excess of non-synonymous mutations. Despite deep divergence between two major allelic lineages, functional diversity was surprisingly low (3 supertypes). Overall, these findings suggest the role of positive and balancing selection in promotion and long-time maintenance of DAB1 polymorphism. Further investigations will clarify the role of pathogen-mediated selection to drive the evolution of DAB1 variation.

Název v anglickém jazyce

Individual copy number variation and extensive diversity between major MHC-DAB1 allelic lineages in the European bitterling

Popis výsledku anglicky

Polymorphism of the major histocompatibility complex (MHC), DAB1 gene was characterized for the first time in the European bitterling (Rhodeus amarus), a freshwater fish employed in studies of host-parasite coevolution and mate choice, taking advantage of newly designed primers coupled with high-throughput amplicon sequencing. Across 221 genotyped individuals, we detected 1-4 variants per fish, with 28% individuals possessing 3-4 variants. We identified 36 DAB1 variants, and they showed high sequence diversity mostly located within predicted antigen-binding sites, and both global and codon-specific excess of non-synonymous mutations. Despite deep divergence between two major allelic lineages, functional diversity was surprisingly low (3 supertypes). Overall, these findings suggest the role of positive and balancing selection in promotion and long-time maintenance of DAB1 polymorphism. Further investigations will clarify the role of pathogen-mediated selection to drive the evolution of DAB1 variation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10617 - Marine biology, freshwater biology, limnology

Projekt

<a href="/cs/project/GA19-05510S" target="_blank" >GA19-05510S: Individuální variabilita a resilience mezidruhových vztahů ve sladkovodním prostředí: vhled pomocí interakcí mlžů a ryb</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Immunogenetics

ISSN

0093-7711

e-ISSN

1432-1211

Svazek periodika

74

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

497-505

Kód UT WoS článku

000741229700001

EID výsledku v databázi Scopus

2-s2.0-85122650049