Interleukin-10 production by B cells is regulated by cytokines, but independently of GATA-3 or FoxP3 expression

Kód výsledku v IS VaVaI

RIV/68378041:_____/20:00539321 - isvavai.cz

Nalezeny alternativní kódy

RIV/00216208:11310/20:10404020

Výsledek na webu

https://www.sciencedirect.com/science/article/abs/pii/S0008874919301893?via%3Dihub

DOI - Digital Object Identifier

10.1016/j.cellimm.2019.103987

Jazyk výsledku

angličtina

Název v původním jazyce

Interleukin-10 production by B cells is regulated by cytokines, but independently of GATA-3 or FoxP3 expression

Popis výsledku v původním jazyce

The knowledge of mechanisms of regulation of IL-10 production by B cells remains still very limited. We show here that highly purified mouse B cells stimulated with LPS produce significant levels of IL-10, but Bregs in our model do not express detectable level of either Foxp3 or GATA-3. Nevertheless, IL-10 production by B cells is regulated by cytokines. In activated B cells, IL-10 production was significantly enhanced by IFN-gamma and decreased in the presence of IL-4 or TGF-beta. These findings are in sharp contrast with the observations in T cells, where IL-10 production correlates with GATA-3 or FoxP3 expression, and the cytokines regulate IL-10 production in a reverse manner than in activated B cells. These results thus show that the production of IL-10 by Bregs is regulated by cytokines independently of the expression of GATA-3 and FoxP3, which is clearly different from GATA-3-dependent IL-10 production by activated Th2 cells and FoxP3 expression in IL-10-producing Tregs.

Název v anglickém jazyce

Interleukin-10 production by B cells is regulated by cytokines, but independently of GATA-3 or FoxP3 expression

Popis výsledku anglicky

The knowledge of mechanisms of regulation of IL-10 production by B cells remains still very limited. We show here that highly purified mouse B cells stimulated with LPS produce significant levels of IL-10, but Bregs in our model do not express detectable level of either Foxp3 or GATA-3. Nevertheless, IL-10 production by B cells is regulated by cytokines. In activated B cells, IL-10 production was significantly enhanced by IFN-gamma and decreased in the presence of IL-4 or TGF-beta. These findings are in sharp contrast with the observations in T cells, where IL-10 production correlates with GATA-3 or FoxP3 expression, and the cytokines regulate IL-10 production in a reverse manner than in activated B cells. These results thus show that the production of IL-10 by Bregs is regulated by cytokines independently of the expression of GATA-3 and FoxP3, which is clearly different from GATA-3-dependent IL-10 production by activated Th2 cells and FoxP3 expression in IL-10-producing Tregs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cellular Immunology

ISSN

0008-8749

e-ISSN

—

Svazek periodika

347

Číslo periodika v rámci svazku

jan.

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

103987

Kód UT WoS článku

000504759200007

EID výsledku v databázi Scopus

2-s2.0-85075876803