Kompletní nukleotidová sekvence a genomová analýza bakteriofága BFK20, lytického fága průmyslové bakterie Brevibacterium flavum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F06%3A00044701" target="_blank" >RIV/68378050:_____/06:00044701 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Complete nucleotide sequence and genome analysis of bacteriophage BFK20 ? A lytic phage of the industrial producer Brevibacterium flavum

Popis výsledku v původním jazyce

Total DNA genome of bacteriophage BFK20, a lytic phage of Brevibacterium flavum CCM251 ? L-lysine industrial producer ? was sequenced and analyzed. It consists of 42968 base pairs with an overall molar G+C content of 56.2%. Fifty-five potential open reading frames were identified and annotated using various bioinformatics tools. Clusters of functionally related putative genes were defined (structural, lytic, replication and regulatory). To verify the annotation of structural proteins, they were resolvedby 2D gel electrophoresis and were submitted to N-terminal amino acid sequencing. Structural proteins identified included the portal and major and minor tail proteins. Based on the overall genome sequence comparison, similarities with other known bacteriophage genomes include primarily bacteriophages from Mycobacterium spp. and some regions of Corynebacterium spp. genomes ? possible prophages. Our results support the theory that phage genomes are mosaics with respect to each other.

Název v anglickém jazyce

Complete nucleotide sequence and genome analysis of bacteriophage BFK20 ? A lytic phage of the industrial producer Brevibacterium flavum

Popis výsledku anglicky

Total DNA genome of bacteriophage BFK20, a lytic phage of Brevibacterium flavum CCM251 ? L-lysine industrial producer ? was sequenced and analyzed. It consists of 42968 base pairs with an overall molar G+C content of 56.2%. Fifty-five potential open reading frames were identified and annotated using various bioinformatics tools. Clusters of functionally related putative genes were defined (structural, lytic, replication and regulatory). To verify the annotation of structural proteins, they were resolvedby 2D gel electrophoresis and were submitted to N-terminal amino acid sequencing. Structural proteins identified included the portal and major and minor tail proteins. Based on the overall genome sequence comparison, similarities with other known bacteriophage genomes include primarily bacteriophages from Mycobacterium spp. and some regions of Corynebacterium spp. genomes ? possible prophages. Our results support the theory that phage genomes are mosaics with respect to each other.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Virology

ISSN

0042-6822

e-ISSN

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Svazek periodika

348

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

57-71

Kód UT WoS článku

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EID výsledku v databázi Scopus

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