Cyclic changes in the amide bands within Escherichia coli biofilms monitored using real-time infrared attenuated total reflection spectroscopy (IR-ATR)
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F19%3A00519146" target="_blank" >RIV/68378271:_____/19:00519146 - isvavai.cz</a>
Výsledek na webu
<a href="https://doi.org/10.1177/0003702819829081" target="_blank" >https://doi.org/10.1177/0003702819829081</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1177/0003702819829081" target="_blank" >10.1177/0003702819829081</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Cyclic changes in the amide bands within Escherichia coli biofilms monitored using real-time infrared attenuated total reflection spectroscopy (IR-ATR)
Popis výsledku v původním jazyce
Contrary to the planktonic state of bacteria, their biofilm form represents severe complications in areas such as human medicine or food industry due to the increasing resistance against harsh conditions and treatment. In the present study, infrared attenuated total reflection (IR-ATR) spectroscopy has been applied as an analytic tool studying Escherichia coli (E. coli) biofilm formation close to real time. We report on IR spectroscopic investigations on the biofilm formation via ATR waveguides probing the biofilm in the spectral window of 1800–900 cm−1 at dynamic flow conditions, which facilitated monitoring the growth dynamics during several days. Observing this behavior via IR spectroscopy allows revealing these cyclical changes in bottom-layered bacteria within the biofilm under continuous nutrient flow, in molecular detail, and during extended periods for the first time.
Název v anglickém jazyce
Cyclic changes in the amide bands within Escherichia coli biofilms monitored using real-time infrared attenuated total reflection spectroscopy (IR-ATR)
Popis výsledku anglicky
Contrary to the planktonic state of bacteria, their biofilm form represents severe complications in areas such as human medicine or food industry due to the increasing resistance against harsh conditions and treatment. In the present study, infrared attenuated total reflection (IR-ATR) spectroscopy has been applied as an analytic tool studying Escherichia coli (E. coli) biofilm formation close to real time. We report on IR spectroscopic investigations on the biofilm formation via ATR waveguides probing the biofilm in the spectral window of 1800–900 cm−1 at dynamic flow conditions, which facilitated monitoring the growth dynamics during several days. Observing this behavior via IR spectroscopy allows revealing these cyclical changes in bottom-layered bacteria within the biofilm under continuous nutrient flow, in molecular detail, and during extended periods for the first time.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10610 - Biophysics
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Applied Spectroscopy
ISSN
0003-7028
e-ISSN
—
Svazek periodika
73
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
9
Strana od-do
424-432
Kód UT WoS článku
000464420300007
EID výsledku v databázi Scopus
2-s2.0-85062011734