CELL MEMBRANE STIFFNESS AND ADHESION MEASURED BY INSTRUMENTED NANOINDENTATION
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21220%2F15%3A00301027" target="_blank" >RIV/68407700:21220/15:00301027 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.czech-in.org/ESB/ESB2015_Abstract_Book.pdf" target="_blank" >http://www.czech-in.org/ESB/ESB2015_Abstract_Book.pdf</a>
DOI - Digital Object Identifier
—
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
CELL MEMBRANE STIFFNESS AND ADHESION MEASURED BY INSTRUMENTED NANOINDENTATION
Popis výsledku v původním jazyce
Cell adhesion on different substrates as well as mechanical properties of cell membrane itself is one of the main topics in tissue engineering. Fluorescence microscope option for `state of the art’ nanomechanical test instruments enables clear visualization of Green Fluorescent Protein (GFP) transfected cells kept in a nutrient medium solution. However, identification of initial contact between an indenter probe and a very soft cell membrane can be very challenging. Contact forces are very low even compared to PBS meniscus forces and membrane adhesive forces acting on the probe. It has been recently shown that monitoring phase shift during oscillatory approach clearly identifies the contact point.
Název v anglickém jazyce
CELL MEMBRANE STIFFNESS AND ADHESION MEASURED BY INSTRUMENTED NANOINDENTATION
Popis výsledku anglicky
Cell adhesion on different substrates as well as mechanical properties of cell membrane itself is one of the main topics in tissue engineering. Fluorescence microscope option for `state of the art’ nanomechanical test instruments enables clear visualization of Green Fluorescent Protein (GFP) transfected cells kept in a nutrient medium solution. However, identification of initial contact between an indenter probe and a very soft cell membrane can be very challenging. Contact forces are very low even compared to PBS meniscus forces and membrane adhesive forces acting on the probe. It has been recently shown that monitoring phase shift during oscillatory approach clearly identifies the contact point.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
BO - Biofyzika
OECD FORD obor
—
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů