Physico-chemical properties of PDMS surfaces suitable as substratesfor cell cultures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21220%2F16%3A00300557" target="_blank" >RIV/68407700:21220/16:00300557 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0169433216314362" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0169433216314362</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.apsusc.2016.07.009" target="_blank" >10.1016/j.apsusc.2016.07.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Physico-chemical properties of PDMS surfaces suitable as substratesfor cell cultures

Popis výsledku v původním jazyce

Elastic properties of the substrate have profound effect on adhesion and proliferation of cells. Here, weintroduce a method to produce polydimethylsiloxane (PDMS) substrates with stiffness tuned mono-tonically from 1.67 to 0.24 MPa, by the time of UV irradiation adjusted up to 5 h. The Young’s modulus(determined by using nanoindenter) scales linearly with stiffness calculated using AFM-based force spec-troscopy data. Such a relation enables the determination of the Young modulus from AFM force – distancecurves also when the Herz model is not applicable. Our findings demonstrate that surface propertiesof PDMS substrates are not affected by the applied methodology of tuning substrate elasticity. Finally,the colorimetric proliferation assay (MTT) carried out for non-malignant (HCV29) and cancerous (T24)bladder cancer cells depicted a significant contribution of PDMS substrate elasticity to the behavior ofcells. The softer PDMS substrate demonstrated excellent cytocompatibility whereas the stiff one is morecell-repellent.

Název v anglickém jazyce

Physico-chemical properties of PDMS surfaces suitable as substratesfor cell cultures

Popis výsledku anglicky

Elastic properties of the substrate have profound effect on adhesion and proliferation of cells. Here, weintroduce a method to produce polydimethylsiloxane (PDMS) substrates with stiffness tuned mono-tonically from 1.67 to 0.24 MPa, by the time of UV irradiation adjusted up to 5 h. The Young’s modulus(determined by using nanoindenter) scales linearly with stiffness calculated using AFM-based force spec-troscopy data. Such a relation enables the determination of the Young modulus from AFM force – distancecurves also when the Herz model is not applicable. Our findings demonstrate that surface propertiesof PDMS substrates are not affected by the applied methodology of tuning substrate elasticity. Finally,the colorimetric proliferation assay (MTT) carried out for non-malignant (HCV29) and cancerous (T24)bladder cancer cells depicted a significant contribution of PDMS substrate elasticity to the behavior ofcells. The softer PDMS substrate demonstrated excellent cytocompatibility whereas the stiff one is morecell-repellent.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Surface Science

ISSN

0169-4332

e-ISSN

—

Svazek periodika

389

Číslo periodika v rámci svazku

December

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

247-254

Kód UT WoS článku

000384577600030

EID výsledku v databázi Scopus

2-s2.0-84979543891