PDMS substrate stiffness affects the morphology and growth profiles of cancerous prostate and melanoma cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21220%2F15%3A00221130" target="_blank" >RIV/68407700:21220/15:00221130 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S175161611400304X#" target="_blank" >http://www.sciencedirect.com/science/article/pii/S175161611400304X#</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jmbbm.2014.09.020" target="_blank" >10.1016/j.jmbbm.2014.09.020</a>

Jazyk výsledku

angličtina

Název v původním jazyce

PDMS substrate stiffness affects the morphology and growth profiles of cancerous prostate and melanoma cells

Popis výsledku v původním jazyce

A deep understanding of the interaction between cancerous cells and surfaces is particularly important for the design of lab-on-chip devices involving the use of poly-dimethylsiloxane (PDMS). In our studies, the effect of PDMS substrate stiffness on mechanical properties of cancerous cells was investigated in conditions where the PDMS substrate is not covered with any of extracellular matrix proteins. Two human prostate cancer (Du145 and PC-3) and two melanoma (WM115 and WM266-4) cell lines were cultured on two groups of PDMS substrates that were characterized by istinct stiffness, i.e. 0.75MPa and 2.92 MPa. The results showed the strong effect on cellular behavior and orphology. The detailed analysis of chemical and physical properties of substrates revealed that cellular behavior occurs only due to substrate elasticity.

Název v anglickém jazyce

PDMS substrate stiffness affects the morphology and growth profiles of cancerous prostate and melanoma cells

Popis výsledku anglicky

A deep understanding of the interaction between cancerous cells and surfaces is particularly important for the design of lab-on-chip devices involving the use of poly-dimethylsiloxane (PDMS). In our studies, the effect of PDMS substrate stiffness on mechanical properties of cancerous cells was investigated in conditions where the PDMS substrate is not covered with any of extracellular matrix proteins. Two human prostate cancer (Du145 and PC-3) and two melanoma (WM115 and WM266-4) cell lines were cultured on two groups of PDMS substrates that were characterized by istinct stiffness, i.e. 0.75MPa and 2.92 MPa. The results showed the strong effect on cellular behavior and orphology. The detailed analysis of chemical and physical properties of substrates revealed that cellular behavior occurs only due to substrate elasticity.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/TA01010185" target="_blank" >TA01010185: Nové materiály a povrchové vrstvy pro bionický návrh kloubních náhrad</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of the Mechanical Behavior of Biomedical Materials

ISSN

1751-6161

e-ISSN

—

Svazek periodika

41

Číslo periodika v rámci svazku

January

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

13-22

Kód UT WoS článku

000346217400002

EID výsledku v databázi Scopus

2-s2.0-84908301569