Depth Recording Capabilities of Planar High-Density Microelectrode Arrays

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21230%2F09%3A00157920" target="_blank" >RIV/68407700:21230/09:00157920 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Depth Recording Capabilities of Planar High-Density Microelectrode Arrays

Popis výsledku v původním jazyce

We use a planar, CMOS-based microelectrode array (MEA) featuring 3,150 metal electrodes per mm2 and 126 recording channels to record spatially highly resolved extracellular action potentials (EAPs) from Purkinje cells (PCs) in acute cerebellar slices. AnIndependent-Component-Analysis-based (ICA) spike sorter is used to reveal EAPs of single cells at subcellular resolution. Those EAPs are then used to set up a compartment model of a PC. The model is used to make and finetune estimations of the distancebetween MEA surface and PC soma. This distance is estimated using the amplitude-independent part of the shape of the EAPs obtained from recordings. The estimation shows that, in our preparations, we can record from PCs with the center of their soma at approximately 35 ?m and 90 ?m vertical distance to the chip surface.

Název v anglickém jazyce

Depth Recording Capabilities of Planar High-Density Microelectrode Arrays

Popis výsledku anglicky

We use a planar, CMOS-based microelectrode array (MEA) featuring 3,150 metal electrodes per mm2 and 126 recording channels to record spatially highly resolved extracellular action potentials (EAPs) from Purkinje cells (PCs) in acute cerebellar slices. AnIndependent-Component-Analysis-based (ICA) spike sorter is used to reveal EAPs of single cells at subcellular resolution. Those EAPs are then used to set up a compartment model of a PC. The model is used to make and finetune estimations of the distancebetween MEA surface and PC soma. This distance is estimated using the amplitude-independent part of the shape of the EAPs obtained from recordings. The estimation shows that, in our preparations, we can record from PCs with the center of their soma at approximately 35 ?m and 90 ?m vertical distance to the chip surface.

Druh

D - Stať ve sborníku

CEP obor

JB - Senzory, čidla, měření a regulace

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 4th International IEEE EMBS Conference on Neural Engineering

ISBN

978-1-4244-2073-5

ISSN

—

e-ISSN

—

Počet stran výsledku

4

Strana od-do

—

Název nakladatele

IEEE

Místo vydání

Piscataway

Místo konání akce

Antalya

Datum konání akce

29. 4. 2009

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

—