Deregulated Genes and Pathways in Human Embryonic Lung Fibroblasts (HEL 12469) treated with Complex Mixtures of the Air Pollutants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21230%2F11%3A00204063" target="_blank" >RIV/68407700:21230/11:00204063 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/em.20693/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/em.20693/pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Deregulated Genes and Pathways in Human Embryonic Lung Fibroblasts (HEL 12469) treated with Complex Mixtures of the Air Pollutants

Popis výsledku v původním jazyce

Recently, we have demonstrated the genotoxic activities of complex mixtures of organic extracts from urban air particles collected in various localities of the Czech Republic that differ in the extent and sources of air pollution [Topinka et al., Toxicology Lett. 202 (2011) 186- 192]. In this study, human embryonic lung fibroblasts (HEL12469) were employed to assess changes in gene expression profiles induced by complex mixtures of organic air pollutants adsorbed onto respirable air particles (PM2.5) collected by high volume samplers in 4 localities of the Czech Republic (Ostrava-Bartovice, Ostrava-Poruba, Karvina and Trebon). For this purpose, changes in the genome-wide expression profiles induced by extractable organic matter (EOM) from the PM2.5 particles were analyzed at subtoxic EOM concentrations of 10, 30, and 60 ?g EOM/ml. Dose dependent increases of a number of deregulated genes were observed for all EOMs. Multiple transcripts/genes are significantly correlating with B[a]P con

Název v anglickém jazyce

Deregulated Genes and Pathways in Human Embryonic Lung Fibroblasts (HEL 12469) treated with Complex Mixtures of the Air Pollutants

Popis výsledku anglicky

Recently, we have demonstrated the genotoxic activities of complex mixtures of organic extracts from urban air particles collected in various localities of the Czech Republic that differ in the extent and sources of air pollution [Topinka et al., Toxicology Lett. 202 (2011) 186- 192]. In this study, human embryonic lung fibroblasts (HEL12469) were employed to assess changes in gene expression profiles induced by complex mixtures of organic air pollutants adsorbed onto respirable air particles (PM2.5) collected by high volume samplers in 4 localities of the Czech Republic (Ostrava-Bartovice, Ostrava-Poruba, Karvina and Trebon). For this purpose, changes in the genome-wide expression profiles induced by extractable organic matter (EOM) from the PM2.5 particles were analyzed at subtoxic EOM concentrations of 10, 30, and 60 ?g EOM/ml. Dose dependent increases of a number of deregulated genes were observed for all EOMs. Multiple transcripts/genes are significantly correlating with B[a]P con

Druh

O - Ostatní výsledky

CEP obor

JC - Počítačový hardware a software

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů