Global Gene Expression Changes in Human Embryonic Lung Fibroblasts Induced by Organic Extracts From Respirable Air Particles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21230%2F12%3A00187936" target="_blank" >RIV/68407700:21230/12:00187936 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378041:_____/12:00377763 RIV/00027162:_____/12:#0000944 RIV/00216208:11310/12:10127519

Výsledek na webu

<a href="http://www.particleandfibretoxicology.com/content/9/1/1" target="_blank" >http://www.particleandfibretoxicology.com/content/9/1/1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1743-8977-9-1" target="_blank" >10.1186/1743-8977-9-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Global Gene Expression Changes in Human Embryonic Lung Fibroblasts Induced by Organic Extracts From Respirable Air Particles

Popis výsledku v původním jazyce

Background: Recently, we used cell-free assays to demonstrate the toxic effects of complex mixtures of organic extracts from urban air particles (PM2.5) collected in four localities of the Czech Republic (Ostrava-Bartovice, Ostrava-Poruba, Karvina and Trebon) which differed in the extent and sources of air pollution. To obtain further insight into the biological mechanisms of action of the extractable organic matter (EOM) from ambient air particles, human embryonic lung fibroblasts (HEL12469) were treated with the same four EOMs to assess changes in the genome-wide expression profiles compared to DMSO treated controls. Method: For this purpose, HEL cells were incubated with subtoxic EOM concentrations of 10, 30, and 60 ?g EOM/ml for 24 hours and globalgene expression changes were analyzed using human whole genome microarrays (Illumina). The expression of selected genes was verified by quantitative real-time PCR.

Název v anglickém jazyce

Global Gene Expression Changes in Human Embryonic Lung Fibroblasts Induced by Organic Extracts From Respirable Air Particles

Popis výsledku anglicky

Background: Recently, we used cell-free assays to demonstrate the toxic effects of complex mixtures of organic extracts from urban air particles (PM2.5) collected in four localities of the Czech Republic (Ostrava-Bartovice, Ostrava-Poruba, Karvina and Trebon) which differed in the extent and sources of air pollution. To obtain further insight into the biological mechanisms of action of the extractable organic matter (EOM) from ambient air particles, human embryonic lung fibroblasts (HEL12469) were treated with the same four EOMs to assess changes in the genome-wide expression profiles compared to DMSO treated controls. Method: For this purpose, HEL cells were incubated with subtoxic EOM concentrations of 10, 30, and 60 ?g EOM/ml for 24 hours and globalgene expression changes were analyzed using human whole genome microarrays (Illumina). The expression of selected genes was verified by quantitative real-time PCR.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JC - Počítačový hardware a software

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Particle and Fibre Toxicology

ISSN

1743-8977

e-ISSN

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Svazek periodika

23(43)

Číslo periodika v rámci svazku

9:1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

44

Strana od-do

1-44

Kód UT WoS článku

000301574500001

EID výsledku v databázi Scopus

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