Global Gene Expression Changes Induced by Organic Extracts of Air Pollutants in Human Lung Cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21230%2F13%3A00204084" target="_blank" >RIV/68407700:21230/13:00204084 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.toxicology.org/AI/PUB/Tox/2013Tox.pdf" target="_blank" >http://www.toxicology.org/AI/PUB/Tox/2013Tox.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Global Gene Expression Changes Induced by Organic Extracts of Air Pollutants in Human Lung Cells

Popis výsledku v původním jazyce

Many adverse effects of the ambient air pollution have been linked to polycyclic aromatic hydrocarbons (PAHs) occurring in the complex mixture adsorbed onto airborne particles. Besides their genotoxic effect, some of them are known to act via aryl hydrocarbon receptor (AhR)-mediated nongenotoxic and tumor promoting mechanism. This study employed human lung adenocarcinoma cells (A549) to investigace the effect of complex mixture of the air pollutants on the global gene expression changes. We determined whole-genome gene expression profiles (Illumina platform) of cells treated with organic extracts (EOMs) from particulate matter (<2.5 ?m) collected in four localities in the Czech Republic differing in the level of the air pollution. Simultaneously, detailed chemical analysis of EOMs from each lokality was performed. Despite various sources, EOMs exhibited equal qualitative composition although the absolute level of PAHs and other pollutants in the most polluted locality was much higher c

Název v anglickém jazyce

Global Gene Expression Changes Induced by Organic Extracts of Air Pollutants in Human Lung Cells

Popis výsledku anglicky

Many adverse effects of the ambient air pollution have been linked to polycyclic aromatic hydrocarbons (PAHs) occurring in the complex mixture adsorbed onto airborne particles. Besides their genotoxic effect, some of them are known to act via aryl hydrocarbon receptor (AhR)-mediated nongenotoxic and tumor promoting mechanism. This study employed human lung adenocarcinoma cells (A549) to investigace the effect of complex mixture of the air pollutants on the global gene expression changes. We determined whole-genome gene expression profiles (Illumina platform) of cells treated with organic extracts (EOMs) from particulate matter (<2.5 ?m) collected in four localities in the Czech Republic differing in the level of the air pollution. Simultaneously, detailed chemical analysis of EOMs from each lokality was performed. Despite various sources, EOMs exhibited equal qualitative composition although the absolute level of PAHs and other pollutants in the most polluted locality was much higher c

Druh

O - Ostatní výsledky

CEP obor

JC - Počítačový hardware a software

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů