Processing Electrophoreogram Image

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Processing Electrophoreogram Image

Popis výsledku v původním jazyce

Gel electrophoresis is one of the most frequently used methods in life-sciences research. Electrophoresis is a chemical separation method based on the effect of electric field on the particles. In this paper we present our proposed method for evaluatingthe results of gel electrophoresis images - electrophoreogram. The evaluation of the electrophoreogram images, i.e. to determine the molecular weight of divided particles, is commonly used commercial software. Our method segments image, automatically extracts the brightness curves across individual lanes, finds divided substances (bands) in these curves and calculates molecular weight of divided substances. We implemented the method and verified its success in comparison with commercial software on electrophoreogram images acquired using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). We found that the proposed method gives reliable results comparable with the evaluation using commercial software. Our method allow

Název v anglickém jazyce

Processing Electrophoreogram Image

Popis výsledku anglicky

Gel electrophoresis is one of the most frequently used methods in life-sciences research. Electrophoresis is a chemical separation method based on the effect of electric field on the particles. In this paper we present our proposed method for evaluatingthe results of gel electrophoresis images - electrophoreogram. The evaluation of the electrophoreogram images, i.e. to determine the molecular weight of divided particles, is commonly used commercial software. Our method segments image, automatically extracts the brightness curves across individual lanes, finds divided substances (bands) in these curves and calculates molecular weight of divided substances. We implemented the method and verified its success in comparison with commercial software on electrophoreogram images acquired using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). We found that the proposed method gives reliable results comparable with the evaluation using commercial software. Our method allow

Druh

D - Stať ve sborníku

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

BioDat 2012 - Conference on Advanced Methods of Biological Data and Signal Processing

ISBN

978-80-01-05153-5

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

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Název nakladatele

České vysoké učení technické v Praze

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

27. 11. 2012

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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