Persistence of genetic damage in lymphocytes from former uranium miners

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F12%3A00202027" target="_blank" >RIV/68407700:21460/12:00202027 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12110/12:43884456

Výsledek na webu

<a href="http://www.karger.com/Article/FullText/338084" target="_blank" >http://www.karger.com/Article/FullText/338084</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1159/000338084" target="_blank" >10.1159/000338084</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Persistence of genetic damage in lymphocytes from former uranium miners

Popis výsledku v původním jazyce

Lymphocytes from former uranium miners who finished work underground one or more decades ago were analysed with respect to possibly persisting genetic damage induced by their radiation exposure. A modified micronucleus-centromere test was used which determined the frequency of micronucleus-containing binucleate cells after cytochalasin B treatment and the percentage of centromere-free micronuclei, assessed with the help of immunofluorescence labeling of centromere protein B. Whereas the overall frequency of micronucleus-containing cells was not significantly elevated above the level found in a control group, former miners showed a greater percentage of centromere-free micronuclei, i.e. micronuclei containing only acentric fragments. Our results are inexcellent agreement with those of an earlier uranium miner study and lend support to the assumption that genetic damage from alpha radiation can persist for many years after exposure, possibly due to genomic instability. The frequency of

Název v anglickém jazyce

Persistence of genetic damage in lymphocytes from former uranium miners

Popis výsledku anglicky

Lymphocytes from former uranium miners who finished work underground one or more decades ago were analysed with respect to possibly persisting genetic damage induced by their radiation exposure. A modified micronucleus-centromere test was used which determined the frequency of micronucleus-containing binucleate cells after cytochalasin B treatment and the percentage of centromere-free micronuclei, assessed with the help of immunofluorescence labeling of centromere protein B. Whereas the overall frequency of micronucleus-containing cells was not significantly elevated above the level found in a control group, former miners showed a greater percentage of centromere-free micronuclei, i.e. micronuclei containing only acentric fragments. Our results are inexcellent agreement with those of an earlier uranium miner study and lend support to the assumption that genetic damage from alpha radiation can persist for many years after exposure, possibly due to genomic instability. The frequency of

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

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Projekt

<a href="/cs/project/2B08001" target="_blank" >2B08001: Biologická dozimetrie pro osoby profesně vystavené ionizujícímu záření alfa</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cytogenetics and Genome Research

ISSN

1424-8581

e-ISSN

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Svazek periodika

136

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

7

Strana od-do

288-294

Kód UT WoS článku

000304993000008

EID výsledku v databázi Scopus

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